Millipore Sigma Vibrant Logo

MABF546 Anti-CD25 Antibody (human), PE, clone BC96

This mouse monoclonal antibody Anti-CD25 (human), PE & clone BC96 validated for use in Flow Cytometry for the detection of CD25.

Anti-CD25 Antibody (human), PE, clone BC96 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: IL2RA UniProt Number: P01589
View Products on Sigmaaldrich.com
MABF546
25 tests  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Special Offers

      100% Performance Guaranteed

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMPEMonoclonal Antibody
      Description
      Catalogue NumberMABF546
      DescriptionAnti-CD25 Antibody (human), PE, clone BC96
      Alternate Names
      • Interleukin-2 receptor subunit alpha
      • IL-2 receptor subunit alpha
      • IL-2-RA
      • IL-2R subunit alpha
      • IL2-RA
      • TAC antigen
      • p55
      • CD antigen CD25
      Background InformationThe BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells.
      The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on “induced” peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.
      References
      Product Information
      FormatPE
      PresentationPurified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal antibody Anti-CD25 (human), PE & clone BC96 validated for use in Flow Cytometry for the detection of CD25.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      CloneBC96
      Concentration5 µL (0.06 µg)/test
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • IL2RA
      Purification MethodSize Exclusion Chromatography
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      Flow Cytometry Analysis: 0.06 µg of this antibody detected CD25 in one million human peripheral blood lymphocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size25 tests
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF546 04053252963124

      Related Products & Applications

      Related Products

      Catalog Number Description  
      05-170 Anti-IL-2 Rα Antibody, clone 7G7/B6 Show Pricing & Availability
      MABF547 Anti-CD25 Antibody (human), PE-Cy7, clone BC96 Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies