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217620 Anti-CD64 Mouse mAb (10.1)

Anti-CD64, mouse monoclonal, clone 10.1, recognizes the ~72 kDa CD64 protein (FcR1). It is validated for FC, WB, IP, IHC on frozen sections, and for neutralization studies.

Anti-CD64 Mouse mAb (10.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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217620
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
217620-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~72 kDa CD64 protein (FcR1 receptor).
      Catalogue Number217620
      Brand Family Calbiochem®
      References
      ReferencesDougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Negative controlHPB-MLT
      Positive control4937 cells
      Preservative0.5 mg/ml gentamycin sulfate
      Quality LevelMQ100
      Applications
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Neutralization Studies
      Application NotesFlow Cytometry (10 µg/ml)
      Frozen Sections (see comments)
      Immunoblotting (see comments)
      Immunoprecipitation (see comments)
      Neutralization Studies (see comments)
      Application CommentsThe antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x105 cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman rheumatoid synovial fluid cells/monocytes
      ImmunogenHuman
      Clone10.1
      HostMouse
      Purity≥95% by SDS-PAGE
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      217620-100UG 04055977202915

      Documentation

      Anti-CD64 Mouse mAb (10.1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD64 Mouse mAb (10.1) Certificates of Analysis

      TitleLot Number
      217620

      References

      Reference overview
      Dougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      ApplicationFlow Cytometry (10 µg/ml)
      Frozen Sections (see comments)
      Immunoblotting (see comments)
      Immunoprecipitation (see comments)
      Neutralization Studies (see comments)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~72 kDa CD64 (FcR1) protein.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman rheumatoid synovial fluid cells/monocytes
      Clone10.1
      IsotypeIgG₁
      Specieshuman
      Positive control4937 cells
      Negative controlHPB-MLT
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Preservative0.5 mg/ml gentamycin sulfate
      CommentsThe antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x105 cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesDougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.