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MABC1123-100UG Anti-CEACAM5 (CD66e) Antibody, clone M5A

Anti-CEACAM5 (CD66e), clone M5A, Cat. No. MABC1123, is a humanized monoclonal antibody that detects Carcinoembryonic antigen-related cell adhesion molecule 5 and has been tested for use in Flow Cytometry, Immunohistochemistry, and Radioimmunoassay.

Anti-CEACAM5 (CD66e) Antibody, clone M5A MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: CEACAM5, CEA UniProt Number: P06731
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MABC1123-100UG
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IHC, RIAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1123-100UG
      DescriptionAnti-CEACAM5 (CD66e) Antibody, clone M5A
      Alternate Names
      • Carcinoembryonic antigen-related cell adhesion molecule 5
      • Carcinoembryonic antigen
      • Meconium antigen 100
      • CD66e
      Background InformationCarcinoembryonic antigen-related cell adhesion molecule 5 (UniProt: P06731; also known as Carcinoembryonic antigen, CEA, Meconium antigen 100, CD66e) is encoded by the CEACAM5 (also known as CEA) gene (Gene ID: 1048) in human. CEA is a cell surface heavily glycosylated homodimeric glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEA molecules can either be transmembrane or GPI-linked and are differentially expressed between species. CEA is restricted to the apical face of intestinal epithelial cells in the adult, but it has more diffuse distribution during embryonic development and in tumors. It functions as a calcium independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1. CEA participates in diverse physiological processes, including cell adhesion, differentiation, proliferation, and survival, as well as in carcinogenesis and bacterial pathogenesis. CEA molecules from neighboring cells can interact via their respective extracellular N-terminal IgV-like domain and mediate cell-cell adhesion through trans-oligomerization. CEA is synthesized with a signal peptide (aa 1-34) and a propeptide region (aa 686-702) that are cleaved to produce the mature form. It has seven Ig-like domains. Two isoforms of CEA have been reported that are produced by alternative splicing. Clone M5A is a humanized monoclonal antibody that displays high specificity and sub-nanomolar affinity for tumor-associated CEA. (Ref. Yazaki, PJ et al., (2004). Protein Eng. Des Sel. 17(5);481-489.
      References
      Product Information
      FormatPurified
      PresentationPurified humanized monoclonal antibody in PBS with 0.05% sodium azide. This humanized antibody was produced by transfecting the cDNA into murine myeloma NSO host cells for stable expression.
      Applications
      ApplicationAnti-CEACAM5 (CD66e), clone M5A, Cat. No. MABC1123, is a humanized monoclonal antibody that detects Carcinoembryonic antigen-related cell adhesion molecule 5 and has been tested for use in Flow Cytometry, Immunohistochemistry, and Radioimmunoassay.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Radioimmunoassay
      Application NotesImmunohistochemistry Analysis: A representative lot detected CEACAM5 (CD66e) in Immunohistochemistry applications (Nittka, S., et. al. (2014). PLoS One. 9(9):e106921).

      Radioimmunoassay Analysis: A representative lot detected CEACAM5 (CD66e) in Radioimmunoassay applications (Nittka, S., et. al. (2014). PLoS One. 9(9):e106921; Yazaki, P.J., et. al. (2004). Protein Eng Des Sel. 17(5):481-9; Yazaki, P.J., et. al. (2013). Protein Eng Des Sel. 26(3):187-93).
      Biological Information
      ImmunogenFull length purified human Carcinoembryonic antigen-related cell adhesion molecule 5 (CEA).
      CloneM5A
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone M5A is a humanized monoclonal antibody that detects human Carcinoembryonic antigen-related cell adhesion molecule 5 (CEA). Note: please use anti-human secondary antibody for detedtion.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CEACAM5
      • CEA
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight76.80 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HT29 cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected CEACAM5 (CD66e) in one million HT29 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABC1123-100UG 04054839482205