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PC711 Anti-CHIP Rabbit pAb

This Anti-CHIP Rabbit pAb is validated for use in Immunoblotting for the detection of CHIP.

Anti-CHIP Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Carboxyl Terminus of Hcs70-Interacting Protein

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PC711
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Dr, Ht, H, Mk, M, RRbPolyclonal Antibody

Pricing & Availability

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PC711-100UL
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      Description
      OverviewRecognizes the ~35 kDa CHIP protein. May also detect bands at ~43, ~51, and ~70 kDa corresponding to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively.
      Catalogue NumberPC711
      Brand Family Calbiochem®
      SynonymsAnti-Carboxyl Terminus of Hcs70-Interacting Protein
      References
      ReferencesXu, W., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 12847.
      Demand, J., et al. 2001. Curr. Biol. 11, 1569.
      Connell, P., et al. 2001. Nat. Cell Biol. 3, 93.
      Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
      Meacham, G.C., et al. 2001. Nat. Cell. Biol. 3, 100.
      Ballinger, C.A., et al. 1999. Mol. Cell. Biol. 19, 4535.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlCOS-7 cells
      Preservative≤0.1% Sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (1:200-1:1000)
      Immunofluorescence (1:250)
      Immunoprecipitation (1:20)
      Application CommentsMay also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of full-length human CHIP fused to a His•Tag® sequence
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Drosophila
      • Hamster
      • Human
      • Monkey
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      PC711-100UL 04055977207897

      Documentation

      Anti-CHIP Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CHIP Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC711

      References

      Reference overview
      Xu, W., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 12847.
      Demand, J., et al. 2001. Curr. Biol. 11, 1569.
      Connell, P., et al. 2001. Nat. Cell Biol. 3, 93.
      Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
      Meacham, G.C., et al. 2001. Nat. Cell. Biol. 3, 100.
      Ballinger, C.A., et al. 1999. Mol. Cell. Biol. 19, 4535.

      Brochure

      Title
      The Active Siteﾙ Volume 4, Number 1
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-October-2018 JSW
      SynonymsAnti-Carboxyl Terminus of Hcs70-Interacting Protein
      ApplicationImmunoblotting (1:200-1:1000)
      Immunofluorescence (1:250)
      Immunoprecipitation (1:20)
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~35 kDa CHIP protein.
      BackgroundCHIP is a U-box dependent E3 ubiquitin ligase and a co-chaperone protein that interacts with Hsc70 and Hsp90 regulating protein triage decisions between folding and degradation. This occurs through CHIP remodeling the complexes to favor substrate degradation thus providing a molecular link between the chaperone and proteosome machinery. CHIP has been reported to be involved in the degradation of c-ErbB2/c-Neu, the glucocorticoid receptor, and the cystic fibrosis transmembrane-conductance regulator. CHIP expression shortens the half-life of both the nascent and mature ErbB2 protein.
      HostRabbit
      Immunogena recombinant protein consisting of full-length human CHIP fused to a His•Tag® sequence
      IsotypeIgG
      SpeciesDrosophila, hamster, human, monkey, mouse, rat
      Positive controlCOS-7 cells
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% Sodium azide
      CommentsMay also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesXu, W., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 12847.
      Demand, J., et al. 2001. Curr. Biol. 11, 1569.
      Connell, P., et al. 2001. Nat. Cell Biol. 3, 93.
      Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
      Meacham, G.C., et al. 2001. Nat. Cell. Biol. 3, 100.
      Ballinger, C.A., et al. 1999. Mol. Cell. Biol. 19, 4535.