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MABE1878-100UG Anti-CNOT6 Antibody, clone Y6206

Anti-CNOT6, clone Y6206, Cat. No. MABE1878, is a mouse monoclonal antibody that detects CCR4-NOT transcription complex subunit 6 (CNOT6) and is tested for use in Western Blotting.

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MABE1878-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1878-100UG
      DescriptionAnti-CNOT6 Antibody, clone Y6206
      Alternate Names
      • CCR4-NOT transcription complex subunit 6
      • EC:3.1.13.4
      • CCR4 carbon catabolite repression 4-like
      • Carbon catabolite repressor protein 4 homolog
      • Cytoplasmic deadenylase
      Background InformationCCR4-NOT transcription complex subunit 6 (UniProt: Q9ULM6; also known as EC:3.1.13.4, CCR4 carbon catabolite repression 4-like, Carbon catabolite repressor protein 4 homolog, Cytoplasmic deadenylase) is encoded by the Cnot6 (also known as Ccr4, Kiaa1194) gene (Gene ID: 57472) in human species. CNOT6, a catalytic component of the CCR4-NOT complex with in mammals. This complex is ubiquitously expressed and contains at least ten subunits. Subunits CNOT6, CNOT7, and CNOT8 display deadenylase activity and CNOT1, 2, 3, 9, 10, and 11 have regulatory NOT modules. CCR4-NOT complex is reported to limit expression of specific genes through deadenylation of mRNA poly(A) tails, enabling positive selection. CNOT6 is detected both in the nucleus and in cytoplasm. It also serves as a poly(A) nuclease with 3'-5' RNase activity. It has been linked to multiple cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation, and general transcription regulation. The deadenylase activity of CCR4-NOT complex has also been implicated in the control of obesity and energy metabolism. In the liver, the deadenylase activity is recruited to the 3 -end of mRNAs of energy metabolism-associated genes and shortens their lifespans and reduces their expression levels. Subunits CNOT 6, 7, and 8 are also shown to participate in the regulation of induced pluripotent stem cell generation and CNOT1, 2, and 3 are involved in maintenance and self-renewal of embryonic stem cells. (Ref.: Ito-Kureha, T., et al. (2020). Nat. Commun. 11; 6169; Zukeran, A., et al. (2016). Biochem. Biophys, Res. Commun. 474; 233-239; Takahashi, A., et al. (2015). Cell Reports 13, 2756-2767).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CNOT6, clone Y6206, Cat. No. MABE1878, is a mouse monoclonal antibody that detects CCR4-NOT transcription complex subunit 6 (CNOT6) and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CNOT6 in lysates from Mouse embryonic stem cells and DLD-1 cells.

      Western Blotting Analysis: A representative lot detected CNOT6 in Western Blotting applications (Ito-Kureha, T., et al. (2020). Nat Commun.11(1):6169; Otsuka, H., et al. (2020). Biochimie.;174:49-56; Mostafa, D., et al. (2020). RNA Biol.;17(3):403-416; Zukeran, A., et al. (2016). Biochem Biophys Res Commun. 474(2):233-239; Takahashi, A., et al. (2015). Cell Rep. 13(12):2756-67).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenA recombinant fragment corresponding to 50 amino acids from the N-terminal half of murine CNOT6 fused with baculovirus gp64 protein.
      EpitopeN-terminal half
      CloneY6206
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone Y6202 is a mouse monoclonal antibody that detects CCR4-NOT transcription complex subunit 6 (CNOT6). It targets an epitope within the N-terminal half.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cnot6
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~62 kDa observed; 63.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CNOT6 in HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE1878-100UG 04065267404028