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ABS2202 Anti-Cas9

Anti-Cas9 Antibody, Cat. No. ABS2202, is a highly specific rabbit polyclonal antibody that targets Cas9 and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-Cas9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Cas9, Csn1 UniProt Number: Q99ZW2
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ABS2202
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      BactICC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS2202
      DescriptionAnti-Cas9
      Alternate Names
      • SpCas9
      • CRISPR-associated endonuclease Cas9
      Background InformationCRISPR-associated endonuclease Cas9/Csn1 (UniProt: Q99ZW2; also known as SpCas9, SpyCas9) is encoded by the Cas9 (also known as Csn1) gene (Gene ID: 901176) in Streptococcus pyogenes. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bi-lobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. The homologous region of the Staphylococcus aureus is not closely related in amino acid sequence in Streptococcus pyogenes, hence this antibody does not recognize that protein.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cas9 Antibody, Cat. No. ABS2202, is a highly specific rabbit polyclonal antibody that targets Cas9 and has been tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cas9 in HEK293 cells transfected with Cas9 from Streptococcus pyogenes.

      Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected Cas9 in non-transfected HEK293H and HEK293H transfected with pCDNA3.0-Cas9.

      Western Blotting Analysis: 10 µg/mL from a representative lot detected Cas9 in HEK293H cell lysate from cells transfected with pCDNA3.0-Cas9.
      Biological Information
      ImmunogenKLH-conjugated linenar peptide corresponding to 18 amino acids from the C-terminal region of Streptococcus pyogenes.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Cas9 in Streptococcus pyogenes. It targets an epitope within 18 amino acids from the C-terminal region. This antibody does not react with Cas9 from Staphylococcus aureus.
      Species Reactivity
      • Bacteria
      Species Reactivity NoteStreptococcus pyogenes.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cas9
      • Csn1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight158.44 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cells transfected with Cas9 from Streptococcus pyogenes.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Cas9 in HEK293 cells transfected with Cas9 from Streptococcus pyogenes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS2202 04054839355561