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MABS1291 Anti-Cas9 Antibody, C-term. clone 10C11-A12

Anti-Cas9 Antibody, C-term. clone 10C11-A12 is an antibody against Cas9 for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-Cas9 Antibody, C-term. clone 10C11-A12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: cas9, csn1 UniProt Number: Q99ZW2
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MABS1291
100 μg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      BactICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1291
      DescriptionAnti-Cas9 Antibody, C-term. clone 10C11-A12
      Alternate Names
      • CRISPR-associated endonuclease Cas9/Csn1
      • SpyCas9
      Background InformationCRISPR-associated endonuclease Cas9/Csn1 (UniProt Q99ZW2; also known as SpyCas9) is encoded by the Streptococcus pyogenes serotype M1 gene cas9 (also known as csn1) gene (Gene ID 901176). Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA loci containing short repetitions of base sequences complementary to antecedent mobile elements and target invading nucleic acids. Each repetition is followed by short segments of spacer DNA from previous exposures to a virus. Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1's CRISPR immune system that confers resistance to foreign genetic elements such as plasmids and phages. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems, correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and Cas9. Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3'-5' exonucleolytically.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cas9 Antibody, C-term. clone 10C11-A12 is an antibody against Cas9 for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: 33.5 µg/mL from a representative lot detected doxycycline-induced expression of exogenously transfected S. pyogenes Cas9 construct under the control of the PTight (Tet-ON) promoter by fluorescent immunocytochemistry staining of methanol-fixed, acetone-permeabilized HeLa transfectants (Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).
      Immunoprecipitation Analysis: 13.4 µg from a representative lot immunoprecipitated exogenously expressed Cas9 in 250 µg of lysate from transfected, but not untransfected, HeLa cells (Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).
      Western Blotting Analysis: 1.675 µg/mL from a representative lot detected exogenously expressed Cas9 in 20 µg of lysate from transfected, but not untransfected, HeLa cells (Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).
      Western Blotting Analysis: Clone 10C11-A12 hybridoma culture supernatant detected doxycycline treatment-induced expression of a FLAG-tagged Cas9 construct under the control of the PTight promoter in a HeLa-derived Tet-ON cell line (Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).
      Biological Information
      ImmunogenHis-tagged recombinant S.pyogenes Cas9 C-terminal fragment.
      EpitopeC-terminal region.
      Clone10C11-A12
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 10C11-A12 recognizes an epitope located in the C-terminal region of Cas9.
      IsotypeIgG1κ
      Species Reactivity
      • Bacteria
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • cas9
      • csn1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~158 kDa observed. 158.4 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Cas9-transfected HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected the exogenously expressed Cas9 in 10 µg lysate from transfected HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS1291 04055977335224

      Documentation

      Anti-Cas9 Antibody, C-term. clone 10C11-A12 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cas9 Antibody, C-term. clone 10C11-A12 Certificates of Analysis

      TitleLot Number
      Anti-Cas9, C-term. Clone 10C11-A12 - 3438027 3438027
      Anti-Cas9, C-term. Clone 10C11-A12 - 3541410 3541410
      Anti-Cas9, C-term. Clone 10C11-A12 - 3905431 3905431
      Anti-Cas9, C-term. Clone 10C11-A12 -2802543 2802543
      Anti-Cas9, C-term. Clone 10C11-A12 -Q2671475 Q2671475
      Anti-Cas9, C-term. Clone 10C11-A12 Monoclonal Antibody 2930963
      Anti-Cas9, C-term. Clone 10C11-A12 Monoclonal Antibody 3009983
      Anti-Cas9, C-term. Clone 10C11-A12 Monoclonal Antibody 2907824
      Anti-Cas9, C-term. Clone 10C11-A12 Monoclonal Antibody 3099062
      Anti-Cas9, C-term. Clone 10C11-A12 Monoclonal Antibody - 4042766 4042766