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MAC142 Anti-Cas9 Antibody, N-terminal Antibody, clone 6H4 Antibody, S. aureus

Anti-Cas-9, N-terminal, clone 6H4, Cat. No. MAC142, is a mouse monoclonal antibody that detects Cas-9 in Staphylococcus aureus and its identity has been confimed by Isotyping Test and Western Blotting.

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MAC142
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAC142
      DescriptionAnti-Cas9 Antibody, N-terminal Antibody, clone 6H4 Antibody, S. aureus
      Alternate Names
      • SaCas9
      • CRISPR-associated endonuclease Cas-9
      Background InformationCRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9) is encoded by the Cas9 gene (Gene ID: in Staphylococcus aureus. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer.Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self-versus non-self, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity. Cas9 endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3'-5' exonucleolytically.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cas-9, N-terminal, clone 6H4, Cat. No. MAC142, is a mouse monoclonal antibody that detects Cas-9 in Staphylococcus aureus and its identity has been confimed by Isotyping Test and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: 0.57 µg/mL from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in whole cell lysates of HEK293 (WT), HEK293/Cas9 from S. aureus, or HeLa/Cas9 from S. pyogenes. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

      Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in transiently transfected U20S cells expressing SaCas9-T2A-GFP. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

      Immunoprecipitation Analysis: 1-2 µg from a representative lot detected CRISPR-associated endonuclease Cas9/Csn1 in SaCas9. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
      Biological Information
      ImmunogenHis-tagged recombiant Cas-9 from Staphylococcus aureus.
      EpitopeN-terminus
      Clone6H4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6H4 detects Cas-9 in Staphylococcus aureus.
      IsotypeIgG2bκ
      Species Reactivity
      • Bacteria
      • All
      Species Reactivity NoteStaphylococcus aureus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Cas9
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~124 kDa observed; 123.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIdentity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAC142 04054839233135

      Documentation

      Anti-Cas9 Antibody, N-terminal Antibody, clone 6H4 Antibody, S. aureus SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cas9 Antibody, N-terminal Antibody, clone 6H4 Antibody, S. aureus Certificates of Analysis

      TitleLot Number
      Anti-Cas9, N-terminal, clone 6H4, S. aureus Monoclonal Antibody 3078409
      Anti-Cas9, N-terminal, clone 6H4, S. aureus - 3455241 3455241
      Anti-Cas9, N-terminal, clone 6H4, S. aureus Monoclonal Antibody Q2979665
      Anti-Cas9, N-terminal, clone 6H4, S. aureus Monoclonal Antibody Q2871721

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