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MABF918 Anti-EAT-2 Antibody, clone 8F12

Anti-EAT-2, clone 8F12, Cat. No. MABF918, is a rat monoclonal antibody that detects SH2 domain-containing protein 1B (EAT-2) and has been tested for use in Western Blotting and Immunoprecipitation.

Anti-EAT-2 Antibody, clone 8F12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Sh2d1b, Eat2, Eat2a, Sh2d1b1 UniProt Number: O35324
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MABF918
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF918
      DescriptionAnti-EAT-2 Antibody, clone 8F12
      Alternate Names
      • SH2 domain-containing protein 1B
      • EWS/FLI1-activated transcript 2
      Background InformationSH2 domain-containing protein 1B (UniProt: O35324; also known as EWS/FLI1-activated transcript 2, EAT-2) is encoded by the Sh2d1b (also known as Eat2, Eat2a, Sh2d1b1) gene in murine species. EAT-2 is a Src homology 2 (SH2) - domain-containing (aa 5-101) intracellular adaptor protein that is related to signaling lymphocytic activation molecule (SLAM) associated protein (SAP). EAT-2 and SAP are expressed in natural killer (NK) cells, and their combined expression appears to be essential for NK cells to kill abnormal hematopoietic cells. NK cells lacking either SAP or EAT-2 display partial defects in NK cell-mediated cytotoxicity toward abnormal hematopoietic cells and more severe defects are observed when NK cells lacking both adaptors. It plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4. It is proposed to act as an inhibitor of CD244-mediated NK cell function including cytotoxicity and interferon-gamma production. It is reported to repress natural cytotoxicity and interferon secretion by a mechanism involving tyrosine phosphorylation of its C-terminus. It binds to the phosphorylated receptors CD84, SLAMF1, LY9 and CD244 and does not bind to non-phosphorylated SLAMF1. Mice lacking EAT-2 are reported to have enhanced NK cell activation responses. EAT-2 is expressed at all stages of maturation in NK cells and in macrophages and dendritic cells. EAT-2 is also expressed in spleen, thymus, lung, kidney, heart, colon and small bowel. (Ref.: Luis-Alberto Pérez-Quintero, L-A., et al. (2014). J. Exp. Med. 211(4); 727-742).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-EAT-2, clone 8F12, Cat. No. MABF918, is a rat monoclonal antibody that detects SH2 domain-containing protein 1B (EAT-2) and has been tested for use in Western Blotting and Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated EAT-2 (Perez-Quintero, L.A., et. al. (2014). J Exp Med. 211(4):727-42).

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected EAT-2 in YT-S transfectants expressing mouse EAT-2 and YT-S wild type cell lysates (Courtesy of Ming-Chao Zhong, Research Associate for Dr. Andre Veillette, Laboratory of Molecular Oncology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada.).

      Western Blotting Analysis: A representative lot detected EAT-2 in Western Blotting applications (Cruz-Munoz, M.E., et. al. (2009). Nat Immunol. 10(3):297-305; Guo, H., et. al. (2009). Mol Cell Biol. 35(1):41-51; Perez-Quintero, L.A., et. al. (2014). J Exp Med. 211(4):727-42.
      Biological Information
      ImmunogenGST-tagged full length recombinant murine SH2 domain-containing protein 1B (EAT-2).
      Clone8F12
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 8F12 detects EWS/FLI1-activated transcript 2 (EAT-2) in human and murine cells.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Sh2d1b
      • Eat2
      • Eat2a
      • Sh2d1b1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight15.26 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be rat IgG2a.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF918 04054839415234