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ABS2237-25UG Anti-EFCAB9

Anti-EFCAB9, Cat. No. ABS2237, is a rabbit polyclonal antibody that detects EF-hand calcium-binding domain-containing protein 9 (EFCAB9) and is tested for use in Immunocytochemistry and Western Blotting.

ABS2237-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2237-25UG
      DescriptionAnti-EFCAB9
      Alternate Names
      • EF-hand calcium-binding domain-containing protein 9
      Background InformationEF-hand calcium-binding domain-containing protein 9 (UniProt: Q9DAM2; also known as EFCAB9) is encoded by the Efcab9 gene (Gene ID: 69306) in murine species. EFCAB9 is an evolutionarily conserved, testis-specific protein that is expressed after meiosis. It serves as a bifunctional cytoplasmic machine that modulates the channel activity and the domain organization of CatSper. It is a pH-dependent calcium sensor that is required for activation of CatSper channel required for sperm cell hyperactivation. This hyperactivation is essential for sperm motility and fertilization. It associates with the CatSper complex via direct interaction with subunit CATSPERz, and senses intracellular Calcium levels. However, it dissociates at elevated pH. It is shown that EFCAB9-CatSperz protein pairs interact with the channel pore as a gatekeeper before capacitation, but it activates the channel in response to capacitation-associated intracellular pH and changes in calcium levels. ECFCAB9 is reported to contain three conserved EF-hand Calcium binding domains. Mice deficient in EFCAB9 display sperm motility defects and compromised fertility. (Ref.: Hwang, JY., et al., (2019). 177(6); 1480-1494).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 10 mM HEPES, 140 mM NaCl, 50% glycerol, pH 7.5, with 50% glycerol.
      Quality LevelMQ200
      Applications
      ApplicationAnti-EFCAB9, Cat. No. ABS2237, is a rabbit polyclonal antibody that detects EF-hand calcium-binding domain-containing protein 9 (EFCAB9) and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected EFCAB9 in Immunocytochemistry applications (Hwang, J.Y., et. al. (2019). Cell. 177(6):1480-1494).

      Western Blotting Analysis: A representative lot detected EFCAB9 in Western Blotting applications (Hwang, J.Y., et. al. (2019). Cell. 177(6):1480-1494).

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected EFCAB9 in mouse sperm lysate, but not in Efcab9 knockout mouse sperm lysate (Courtesy of Dr. Jean-Ju Chung @ Yale University).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the EF-hand 3, C-terminal half of mouse EF-hand calcium-binding domain-containing protein 9 (EFCAB9).
      EpitopeC-terminal half
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects EF-hand calcium-binding domain-containing protein 9 (EFCAB9). It targets an epitope within 18 amino acids from the EF-hand 3 in the C-terminal half.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Efcab9
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~23 kDa observed; 26.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse sperm lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EF-hand calcium-binding domain-containing protein 9 (EFCAB9) in wild-type, but not in EFCAB9 knockout mouse sperm lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS2237-25UG 04065265398145