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ABN423 Anti-Equilibrative Nucleoside Transporter 1 Antibody

This Anti-Equilibrative Nucleoside Transporter 1 antibody is validated for use in western blotting & IHC (Paraffin) for the detection of Equilibrative Nucleoside Transporter 1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: SLC29A1, ENT1 UniProt Number: Q99808
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ABN423
100 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN423
      ReplacesAB15267
      DescriptionAnti-Equilibrative Nucleoside Transporter 1 Antibody
      Alternate Names
      • Equilibrative nucleoside transporter 1
      • Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
      • Equilibrative NBMPR-sensitive nucleoside transporter
      • Nucleoside transporter, es-type
      • Solute carrier family 29 member 1
      Background InformationEquilibrative Nucleoside Transporter 1 (ENT1), also known as Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, Equilibrative NBMPR-sensitive nucleoside transporter, Nucleoside transporter, es-type, or Solute carrier family 29 member 1, and encoded by the gene SLC29A1/ENT1, is a transport membrane protein that is responsible for mediating the transport of nucleoside molecules in and out of cells. Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. ENT1 is spatially localized to the basolateral cell membranes and is found expressed in many organs including heart, brain, mammary glands, liver and spleen. It is up regulated in cancer cells but it is also an anti-cancer target as it is sensitive to the chemotherapeutic drugs dipyridamole and dilazep.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Equilibrative Nucleoside Transporter 1 antibody is validated for use in western blotting & IHC (Paraffin) for the detection of Equilibrative Nucleoside Transporter 1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected Equilibrative Nucleoside Transporter 1 in human cerebellum, human breast cancer, and human cerebral cortex tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Equilibrative Nucleoside Transporter 1. Immunogen is 80% identical to mouse and rat ENT1.
      Epitopecytoplasmic domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Non-Human Primate based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLC29A1
      • ENT1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~50 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human heart tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Equilibrative Nucleoside Transporter 1 in 10 µg of human heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABN423 04053252959219