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ABS2208-100UG Anti-GPR161

Anti-GPR161, Cat. No. ABS2208, is a rabbit polyclonal antibody that detects GPR161 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

ABS2208-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2208-100UG
      DescriptionAnti-GPR161
      Alternate Names
      • G-protein coupled receptor 161
      Background InformationG-protein coupled receptor 161 (UniProt: B2RPY5; also known as GPR161) is encoded by the Gpr161 (also known as Gm208) gene (Gene ID: 240888) in murine species. GPR161 is a multi-pass membrane glycoprotein of the GPCR family with seven transmembrane domains. It localizes to the ciliary membrane and is also detected in the recycling endocytic compartment. It is ubiquitously expressed from 8.5 dpc and is mostly concentrated in the developing nervous system at later stages. By 10.5 dpc, it is mainly expressed in the neural tube. At later embryonic stages it is predominantly expressed in the brain, spinal cord, and dorsal ganglia. GPR161 functions as a negative regulator of sonic hedgehog (Shh) signaling, which promotes the processing of Gli3 into its repressive form (Gli3R) during neural tube development via cAMP-protein kinase A signaling. In the presence of Shh, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity, and allowing activation of the Shh signaling. Gpr161 knockout mice display increased Shh signaling and exhibit embryonic lethality by 10.5 dpc. GPR161 is overexpressed in triple-negative breast cancer and serves is a regulator of mammary epithelial cell proliferation and invasion in a mTOR-dependent manner. Knockdown of GPR161 is reported to impair proliferation of human basal breast cancer cell lines and its overexpression increases cell proliferation, migration, and intracellular accumulation of E-cadherin. Three isoforms of GPR161 have been described that are produced by alternative splicing. (Ref.: Hwang, S-H., et al. (2021). eLife 10:e67121; Mukhopadhyay, S., et al. (2013). Cell 152(1-2); 210-223; Feigin, ME., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(11); 4191-4196).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-GPR161, Cat. No. ABS2208, is a rabbit polyclonal antibody that detects GPR161 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunofluorescence
      • Immunocytochemistry
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected GPR161 in Immunofluorescence application (Shimada, H., et. al. (2017). Cell Rep. 20(2):384-396).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated GPR161 in Immunoprecipitation application (Mukhopadhyay, S., et. al. (2013). Cell. 152(1-2):210-23).

      Immunocytochemistry Analysis: A representative lot detected GPR161 in Immunocytochemistry application (Mukhopadhyay, S., et. al. (2013). Cell. 152(1-2):210-23).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal, cytoplasmic domain of mouse G-protein coupled receptor 161 (GPR161).
      EpitopeC-terminal
      Concentration0.5 mg/mL Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects G-protein coupled receptor 161 (GPR161). It targets an epitope within 28 amino acids from the C-terminal, cytoplasmic domain.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gpr161
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~60 kDa observed; 60.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293T cell transiently overexpressing human GPR161

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GPR161 in lysate from HEK293T cell transiently overexpressing human GPR161.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS2208-100UG 04065270453662