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MAC111 Anti-GST•Tag™ fusion protein Antibody

This Anti-GST•Tag fusion protein Antibody is validated for use in Western Blotting, ICC, Immunoprecipitation, ELISA for the detection of GST•Tag fusion protein.

Anti-GST•Tag™ fusion protein Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epitope Tags & General Use Research Sub-Category: Epitope Tags UniProt Number: P08515
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MAC111
50 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWB, ICC, IP, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAC111
      DescriptionAnti-GST•Tag™ fusion protein Antibody
      Alternate Names
      • Glutathione S-transferase class-mu 26 kDa isozyme
      • GST 26
      • Sj26 antigen
      • SjGST
      Background InformationGST (glutathione-S-transferase) represents a major group of detoxification enzymes, which are known to exist as multiple cytosolic and membrane-bound isoenzymes, with distinct catalytic and noncatalytic binding properties. Whereas the cytosolic enzymes are encoded by at least five distantly related gene families, the membrane-bound enzymes are encoded by single genes. GST is regulated and induced by a diverse range of xenobiotics. Many, but not all, inducers of GST, act through either the antioxidant-responsive element (ARE), the GST P enhancer 1(GPE), the xenobiotic-responsive element (XRE), or the glucocorticoid-responsive element (GRE). Barbiturates may transcriptionally activate GST through a Barbie box element. Many of the compounds that induce GST are also GST substrates, or are metabolized by cytochrome P-450 monooxygenases into compounds that can serve as GST substrates. Because the expression levels of GST play a critical role in determining the sensitivity of cells to a broad range of toxic chemicals, GST is suggested to contribute to resistance against carcinogens, antitumor drugs, environmental pollutants, and products of oxidative stress.
      References
      Product Information
      FormatPurified
      Control
      • Posi-Tag Epitope Tag Control
      PresentationPurified mouse monoclonal IgG1κ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-GST•Tag fusion protein Antibody is validated for use in Western Blotting, ICC, Immunoprecipitation, ELISA for the detection of GST•Tag fusion protein.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      • ELISA
      Application NotesImmunocytochemistry Analysis: A representative lot detected GST•Tag fusion protein in transfected COS-1 cells.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated GST•Tag fusion protein sequence in IP.

      ELISA Analysis: A representative lot detected GST•Tag protein sequence in ELISA.
      Biological Information
      ImmunogenGluththione-S-transferase (GST) domain from Schistosoma Japonicum
      Clone8-326.6.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~45 kDa observed. The molecular weight of the GST•Tag fusion protein is not ~45 kDa. The Posi-Tag Epitope Tag Control, which contains the fusion protein, is observed at ~45 kDa in Western Blot.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Posi-Tag Epitope Tag Control.

      Western Blot Analysis: This antibody detected GST•Tag fusion protein in 5 µL of Posi-Tag Epitope Tag Control.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAC111 04053252550966

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies