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ABS1488 Anti-GTP cyclohydrolase 1

Anti-GTP cyclohydrolase 1, Cat. No. ABS1488, is a rabbit polyclonal antibody that detectsGTP cyclohydrolase 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-GTP cyclohydrolase 1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: GCH1, DYT5, GCH UniProt Number: P30793
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ABS1488
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1488
      DescriptionAnti-GTP cyclohydrolase 1
      Alternate Names
      • EC: 3.5.4.16
      • GTP cyclohydrolase
      • GTP-CH-I
      Background InformationGTP cyclohydrolase 1 (UniProt: P30793; also known as EC: 3.5.4.16, GTP cyclohydrolase, GTP-CH-I) is encoded by the GCH1 (also known as DYT5, GCH) gene (Gene ID: 2643) in human. GTP-CH-1 is a functional enzyme that is involved in step 1 of the subpathway for synthesis of 7, 8-dihydroneopterin triphosphate from GTP. It is shown to positively regulate nitric oxide synthesis in umbilical vein endothelial cells. It is expressed in epidermis and its expression is high in basal undifferentiated keratinocytes. Four isoforms of GTP-CH-1 are reported that are produced by alternative splicing. Its activity is inhibited by tetrahydrobiopterin and GTP has a positive allosteric effect on its activity. It contains three zinc binding sites (aa 141, 144, 211) and Zn2+ is shown to be essential for its catalytic activity. Its activity is also up-regulated by IFN-gamma, TNF, interleukin-1 beta, bacterial lipopolysaccharides and phenylalanine, and is down-regulated by dibutyryl-cAMP, iloprost and 8-bromo-cGMP in HUVEC. Up-regulation of its activity stimulates the production of tetrahydrobiopterin that results in elevation of endothelial nitric oxide synthase activity. GTP-CH-1 is phosphorylated by casein kinase II at serine 81 in aortic endothelial cells during oscillatory shear stress that results in increased enzyme activity. Mutations in GCH1 gene can result in hyperphenylalaninemia and depletion of dopamine and serotonin resulting in psychomotor retardation, tonicity disorders, convulsions, and abnormal movements.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GTP cyclohydrolase 1, Cat. No. ABS1488, is a rabbit polyclonal antibody that detectsGTP cyclohydrolase 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected GTP cyclohydrolase 1 in human vagina and human small intestine tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human GTP cyclohydrolase 1. The immunogen sequence is conserved in all four isoforms.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects GTP cyclohydrolase 1 in human cells. It targets an epitope within 15 amino acids from the N-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • GCH1
      • DYT5
      • GCH
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~28 kDa observed; 27.90 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human liver tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected GTP cyclohydrolase 1 in 10 µg of human liver tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS1488 04054839399749