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393206 Anti-HNE Fluorophore Rabbit pAb

Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.

Anti-HNE Fluorophore Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore

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393206
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesRbPolyclonal Antibody

Pricing & Availability

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393206-100UL
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      		 Glass bottle 100 ul
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      Description
      OverviewRecognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.
      Catalogue Number393206
      Brand Family Calbiochem®
      SynonymsAnti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore
      References
      ReferencesTsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7975.
      Product Information
      FormLiquid
      FormulationSerum diluted 1:1 with glycerol.
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesELISA (1:500-1:1000)
      Immunoblotting (1:1000-1:5000)
      Immunohistochemistry (1:100-1:500)
      Application CommentsDue to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      393206-100UL 04055977212488

      Documentation

      Anti-HNE Fluorophore Rabbit pAb Certificates of Analysis

      TitleLot Number
      393206

      References

      Reference overview
      Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7975.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-July-2007 JSW
      SynonymsAnti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore
      ApplicationELISA (1:500-1:1000)
      Immunoblotting (1:1000-1:5000)
      Immunohistochemistry (1:100-1:500)
      DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.
      HostRabbit
      Immunogena 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH
      IsotypeIgG
      Speciesa broad range of species
      FormLiquid
      FormulationSerum diluted 1:1 with glycerol.
      CommentsDue to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesTsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7975.