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MABN2416 Anti-HSD17B10 Antibody, clone 5F3

Anti-HSD17B10 antibody, clone 5F3, Cat. No. MABN2416, is a highly specific mouse monoclonal antibody that targets HSD17B10 and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-HSD17B10 Antibody, clone 5F3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: HSD17B10, ERAB, HADH2, MRPP2, SCHAD, SDR5C1, XH98G2 UniProt Number: Q99714
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MABN2416
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IH(P), EMMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2416
      DescriptionAnti-HSD17B10 Antibody, clone 5F3
      Alternate Names
      • 3-hydroxyacyl-CoA dehydrogenase type-2
      • 17-beta-hydroxysteroid dehydrogenase 10
      • 17-beta-HSD 10
      • 3-hydroxy-2-methylbutyryl-CoA dehydrogenase
      • 3-hydroxyacyl-CoA dehydrogenase type II
      • Endoplasmic reticulum-associated amyloid beta-peptide-binding protein
      • Mitochondrial ribonuclease P protein 2
      • Mitochondrial RNase P protein 2
      • Short-chain type dehydrogenase/reductase XH98G2
      • Type II HADH
      Background Information3-hydroxyacyl-CoA dehydrogenase type-2 (UniProt: Q99714; also known as 17-beta-hydroxysteroid dehydrogenase 10, 17-beta-HSD 10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 3-hydroxyacyl-CoA dehydrogenase type II, Endoplasmic reticulum-associated amyloid beta-peptide-binding protein, Mitochondrial ribonuclease P protein 2, Mitochondrial RNase P protein 2, Short chain dehydrogenase/ reductase family 5C member 1, Short-chain type dehydrogenase/reductase XH98G2, Type II HADH) is encoded by the HSD17B10 (also known as ERAB, HADH2, MRPP2, SCHAD, SDR5C1, XH98G2) gene (Gene ID: 3028) in human. 3-hydroxyacyl-CoA dehydrogenase type-2 is a part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. It functions in mitochondrial tRNA maturation. It catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. It catalyzes the third step in the beta-oxidation of fatty acids and also carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. With C21 steroids it exhibits 20-beta-OH and 21-OH dehydrogenase activities. May contribute to neuronal dysfunction associated with Alzheimer disease (AD) via interactions with intracellular amyloid-beta peptides. 3-hydroxyacyl-CoA dehydrogenase type-2 is ubiquitously expressed in normal tissues, but is overexpressed in neurons affected in AD.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-HSD17B10 antibody, clone 5F3, Cat. No. MABN2416, is a highly specific mouse monoclonal antibody that targets HSD17B10 and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Electron Microscopy
      Application NotesElectron Microscopy Analysis: A representative lot detected HSD17B10 in Electron Microscopy applications (Wen, G.Y., et. al. (2002). Brain Res. 954(1):115-22).

      Immunocytochemistry Analysis: A representative lot detected HSD17B10 in Immunocytochemistry applications (Frackowiak, J., et. al. (2001). Brain Res. 907(1-2):44-53).

      Western Blotting Analysis: A representative lot detected HSD17B10 in Western Blotting applications (Frackowiak, J., et. al. (2001). Brain Res. 907(1-2):44-53).

      Immunohistochemistry Analysis: A 1:250-1:1,000 dilution from a representative lot detected HSD17B10 in human placenta and human breast cancer tissues.
      Biological Information
      ImmunogenGST-tagged full length recombinant human 3-hydroxyacyl-CoA dehydrogenase type-2 protein (ERAB/HADH).
      Clone5F3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5F3 detects 3-hydroxyacyl-CoA dehydrogenase type-2 in human and murine cells.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HSD17B10
      • ERAB
      • HADH2
      • MRPP2
      • SCHAD
      • SDR5C1
      • XH98G2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~27 kDa observed; 26.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293T cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected HSD17B10 in 10 µg of HEK293T cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABN2416 04054839251016