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ABE1348 Anti-Histone H2A.Z Antibody (C-term)

This Anti-Histone H2A.Z Antibody (C-term) is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Immunocytochemistry and ELISA for the detection of Histone H2A.Z (C-term).

Anti-Histone H2A.Z Antibody (C-term) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H2AFZ, H2AZ UniProt Number: P0C0S5
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ABE1348
50 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ChIP, ChIP-seq, ICC, ELISARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1348
      DescriptionAnti-Histone H2A.Z Antibody (C-term)
      Alternate Names
      • Histone H2A.Z
      • H2A/z
      Background InformationH2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified rabbit polyclonal in PBS containing 0.05% azide and 0.05% ProClin 300.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Histone H2A.Z Antibody (C-term) is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Immunocytochemistry and ELISA for the detection of Histone H2A.Z (C-term).
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      • Immunocytochemistry
      • ELISA
      Application NotesChromatin Immunoprecipitation (ChIP): A representative lot of this antibody (1μg per reaction) was used for ChIP using chromatin from HeLa and K562 cells.

      ChIP-seq: A representative lot of this antibody (0.5 μg per reaction) was used to immunoprecipitate K562 chromatin for ChIP-seq analysis.

      Western Blotting Analysis: A 1:2,000 dilution of a represenative lot of this antibody detected histone H2A.Z in acid extracted HeLa cell lysate.

      Immunocytochemistry: A 1:500 dilution from a representative lot detected histone H2A.Z in HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to region the near C-terminus of Human Histone H2A.Z.
      EpitopeNear C-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityRecognizes histone H2A.Z
      Species Reactivity
      • Human
      Species Reactivity NoteHuman, wide range expected
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H2AFZ
      • H2AZ
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~16 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting using recombinant histones

      Western Blotting Analysis: A 1.5 µg/mL dilution of this antibody detected recombinant Histone H2A.Z, but not histones H1, H2A,H2A.X, H3, or H4.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABE1348 04055977073195

      Documentation

      Anti-Histone H2A.Z Antibody (C-term) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histone H2A.Z Antibody (C-term) Certificates of Analysis

      TitleLot Number
      Anti-Histone H2A.Z (C-term) Q2464803
      Anti-Histone H2A.Z (C-term) - 3226591 3226591
      Anti-Histone H2A.Z (C-term) - 3255344 3255344
      Anti-Histone H2A.Z (C-term) - 3275084 3275084
      Anti-Histone H2A.Z (C-term) - 3310689 3310689
      Anti-Histone H2A.Z (C-term) - 3439804 3439804
      Anti-Histone H2A.Z (C-term) - 3510291 3510291
      Anti-Histone H2A.Z (C-term) - 3574584 3574584
      Anti-Histone H2A.Z (C-term) - 3596866 3596866
      Anti-Histone H2A.Z (C-term) - 3689960 3689960
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies