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386032 Anti-Hsp70 Mouse mAb (C92F3A-5)

Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections).

Anti-Hsp70 Mouse mAb (C92F3A-5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Heat Shock Protein 70

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386032
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Pricing & Availability

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      Description
      OverviewRecognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.
      Catalogue Number386032
      Brand Family Calbiochem®
      SynonymsAnti-Heat Shock Protein 70
      Application Data
      Detection of mouse Hsp70 by immunoblotting immunoblotting. Sample: Whole cell lysate from L929 cells. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:1000). Detection: chemiluminescence.

      Detection of human Hsp70 by staining paraffin sections. Sample: Human colon cancer tissue. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:50). Detection: colorimetric (DAB).

      Substrate binding of Hsp70 in complex with a substrate peptide.
      References
      ReferencesHang, H., and Fox, M.H. 1995. Cytometry 19, 119.
      Kilgore, J.L., et al. 1994. J. Appl. Physiol. 76, 589.
      Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab. 74, 724.
      Gower, D.J., et al. 1989. J. Neurosurg. 70, 605.
      Milarski, K., et al. 1989.J. Cell Biol. 108, 413.
      Vass, K., et al. 1988. Acta Neuropathologica 77, 128.
      Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol. 103, 2035.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Positive controlL929 cells, Human colon cancer tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Paraffin Sections
      Application NotesELISA (see comments)
      Flow Cytometry (see comments)
      Frozen Sections (5 µg/ml)
      Immunoblotting (1 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (see comments)
      Paraffin Sections (5 µg/ml)
      Application CommentsDoes not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      ImmunogenHsp70 from HeLa cells
      ImmunogenHuman
      CloneC92F3A-5
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing iniital thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      386032-50UG 04055977189889

      Documentation

      Anti-Hsp70 Mouse mAb (C92F3A-5) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Hsp70 Mouse mAb (C92F3A-5) Certificates of Analysis

      TitleLot Number
      386032

      References

      Reference overview
      Hang, H., and Fox, M.H. 1995. Cytometry 19, 119.
      Kilgore, J.L., et al. 1994. J. Appl. Physiol. 76, 589.
      Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab. 74, 724.
      Gower, D.J., et al. 1989. J. Neurosurg. 70, 605.
      Milarski, K., et al. 1989.J. Cell Biol. 108, 413.
      Vass, K., et al. 1988. Acta Neuropathologica 77, 128.
      Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol. 103, 2035.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-June-2009 JSW
      SynonymsAnti-Heat Shock Protein 70
      ApplicationELISA (see comments)
      Flow Cytometry (see comments)
      Frozen Sections (5 µg/ml)
      Immunoblotting (1 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (see comments)
      Paraffin Sections (5 µg/ml)
      Application Data
      Detection of mouse Hsp70 by immunoblotting immunoblotting. Sample: Whole cell lysate from L929 cells. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:1000). Detection: chemiluminescence.

      Detection of human Hsp70 by staining paraffin sections. Sample: Human colon cancer tissue. Primary antibody: Anti-Hsp70 Mouse mAb (C92F3A-5) (Cat. No. 386032) (1:50). Detection: colorimetric (DAB).

      Substrate binding of Hsp70 in complex with a substrate peptide.
      DescriptionMouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.
      BackgroundThe Hsp70 family consists of multiple members, all of which bind ATP in vitro, that are localized within different intracellular compartments. Hsp70 is not usually expressed under normal growth conditions, but is expressed at high levels in cells experiencing stress. It is found in the cytosol, nucleus, and nucleolus.
      HostMouse
      Immunogen speciesHuman
      ImmunogenHsp70 from HeLa cells
      CloneC92F3A-5
      IsotypeIgG₁
      Speciesa broad range of species
      Positive controlL929 cells, Human colon cancer tissue
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing iniital thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHang, H., and Fox, M.H. 1995. Cytometry 19, 119.
      Kilgore, J.L., et al. 1994. J. Appl. Physiol. 76, 589.
      Heufelder, A.E., et al. 1992. J. Clin. Endocrinol. Metab. 74, 724.
      Gower, D.J., et al. 1989. J. Neurosurg. 70, 605.
      Milarski, K., et al. 1989.J. Cell Biol. 108, 413.
      Vass, K., et al. 1988. Acta Neuropathologica 77, 128.
      Welch, W.J., and Suhan, J.P. 1986. J. Cell Biol. 103, 2035.