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MABF1478-25UG Anti-IFNGR1/CD119 Antibody, clone 2E2

Anti-IFNGR1/CD119, clone 2E2, Cat. No. MABF1478, is an Armenian hamster monoclonal antibody that detects IFNGR1/CD119 and is tested for use in Flow Cytometry.

NewMABF1478-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF1478-25UG
      DescriptionAnti-IFNGR1/CD119 Antibody, clone 2E2
      Alternate Names
      • Interferon gamma receptor 1
      • IFN-gamma receptor 1
      • IFN-gamma-R1
      • Interferon gamma receptor alpha-chain
      • IFN-gamma-R-alpha
      • CD119
      Background InformationInterferon gamma receptor 1 (UniProt: P15261; also known as IFN-gamma receptor 1, IFN-gamma-R1, Interferon gamma receptor alpha-chain, IFN-gamma-R-alpha, CD119) is encoded by the Ifngr1 (also known as Ifngr) gene (Gene ID: 15979) in murine species. IFNGR1/CD119 is single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 26-254), a transmembrane domain (aa 255-275), and a cytoplasmic domain (aa 276-477). It serves as a receptor subunit for interferon- that plays critical roles in antibacterial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. It associates with IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1/CD119 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, the activated JAK1 phosphorylates IFNGR1/CD119 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to its dimerization, translocation to the nucleus, and stimulation of target gene transcription. Phosphorylation of the intracellular domain of IFNGR1/CD119 also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1. (Ref.: Wei, B., et al. (2010). Am. J. Transplant. 10(1); 69-80; Haring, JS., et al. (2005). J. Immunol. 174(11); 6791-6802; Qing, Y., et al. (2005). J. Biol. Chem. 280(3); 1849-1853).
      References
      Product Information
      FormatPurified
      PresentationPurified armenian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IFNGR1/CD119, clone 2E2, Cat. No. MABF1478, is an Armenian hamster monoclonal antibody that detects IFNGR1/CD119 and is tested for use in Flow Cytometry.
      Key Applications
      • Flow Cytometry
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected IFNGR1/CD119 in Flow Cytometry applications (Haring, J.S., et al. (2005). J Immunol. 174(11):6791-802).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenRecombinant soluble form of -chain of mouse IFNGR1/CD119.
      EpitopeExtracellular domain
      Clone2E2
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostArmenian Hamster
      SpecificityClone 2E2 is an Armenian hamster monoclonal antibody that detects Interferon 1 receptor (IFNGR1/CD119). It targets an epitope within the extracellular domain.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ifngr1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight52.34 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in C57BL/6 Mouse splenocytes.

      Flow Cytometry Analysis: 1.0 µg of this antibody detected IFNGR1/CD119 in one million C57BL/6 Mouse splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF1478-25UG 04065270937919