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ABN2294-25UL Anti-IQSEC3

Anti-IQSEC3, Cat. No. ABN2294, is a rabbit polyclonal antibody that detects IQSEC3 and is tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

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ABN2294-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABN2294-25UL
      DescriptionAnti-IQSEC3
      Alternate Names
      • IQ motif and SEC7 domain-containing protein 3
      • Potential synaptic guanine nucleotide exchange factor for Arf
      • SynArfGEF-Po
      • BRAG3
      Background InformationIQ motif and SEC7 domain-containing protein 3 (UniProt: Q76M68; also known as Potential synaptic guanine nucleotide exchange factor for Arf, SynArfGEF-Po) is encoded by the Iqsec3 (also known as Sag) gene (Gene ID: 404781) in rat. IQSEC3 along with IQSEC1 and IQSEC2 constitutes a family of brefeldin A-resistant ARF guanine nucleotide exchange factors (GEFs). It is a cytoplasmic protein that is expressed in the brain and is localized to dendrites and soma. Its expression is reported as early as E18 in hippocampal neurons. It is a key factor in controlling network activity in the hippocampus under both physiological and pathological conditions. It is shown to be exclusively localized to inhibitory synapses and it directly binds to gephyrin to promote inhibitory synapse formation in an ARF-GEF activity-dependent manner. IQSEC3 is reported to regulate GABAergic synapse maintenance in vivo and it links gephyrin-GABAA receptor complexes with ARF-dependent pathways to prevent aberrant, runaway excitation. Knockdown of Iqsec3 gene in the hippocampal dentate gyrus (DG) induces a significant reduction in GABAergic synapse density and causes a massive reduction of somatostatin expression in the DG hilus. IQ domain of IQSEC3 is localized to amino acids 311-340; the SEC7 domain is in amino acids 646-838; and the PH domain is in amino acids 852-985. (Ref.: Kim, S., et al. (2020). Cell Rep. 30(6); 1995-2005; Um, JW., et al. (2016). J. Biol. Chem. 291(19); 10119-10130).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in 10 mM Tris-HCl pH 7.5 with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IQSEC3, Cat. No. ABN2294, is a rabbit polyclonal antibody that detects IQSEC3 and is tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IQSEC3 in mouse brain tissue lysate.

      Immunohistochemistry Applications: A representative lot detected IQSEC3 in Immunohistochemistry applications (Kim, S., et. al. (2020). Cell Rep. 30(6):1995-2005).

      Immunocytochemistry Analysis: A representative lot detected IQSEC3 in Immunochemistry applications (Um, JW., et. al. (2016). J. Biol. Chem. 291(19):10119-30).

      Immunoprecipitation Analysis: A representative lot detected IQSEC3 in Immunoprecipitation applications (Um, JW., et. al. (2016). J. Biol. Chem. 291(19):10119-30).

      Western Blotting Analysis: A representative lot detected IQSEC3 in lysates from HEK293T cells transfected with Flag-IQSEC3, but not in untransfected cells. (Data Courtesy of Dr Jaewon Ko & Dr Seungjoon Kim at Daegu Gyeongbuk Institute of Science and Technology (DGIST)

      Western Blotting Analysis: A representative lot detected IQSEC3 in Western Blotting applications (Kim, S., et. al. (2020). Cell Rep. 30(6):1995-2005).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the first 185 amino acids from rat IQ motif and SEC7 domain-containing protein 3 (IQSEC3).
      EpitopeN-terminal
      Concentration0.15 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects IQ motif and SEC7 domain-containing protein 3 (IQSEC3). It targets an epitope within the N-terminal domain.
      IsotypeIgG
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteMouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Iqsec3
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~150 kDa observed; 129.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IQSEC3 in Rat brain tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABN2294-25UL 04065268041901