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05-1066-25UG Anti-Insulin Antibody, clone E11D7 (mouse monoclonal)

Anti-Insulin Antibody, clone E11D7 detects level of Insulin & has been published & validated for use in ELISA, IH(P).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling, Metabolism Research Sub-Category: Insulin/Energy Signaling, Glucose/Glycogen Metabolism, Metabolic Hormones & Receptors Gene Symbol: INS, proinsulin, insulin UniProt Number: P01308
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05-1066-25UG
25 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RELISA, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1066-25UG
      DescriptionAnti-Insulin Antibody, clone E11D7 (mouse monoclonal)
      Background InformationInsulin is a polypeptide hormone that regulates carbohydrate metabolism. Apart from being the primary agent in carbohydrate homeostasis, it has effects on fat metabolism.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monolconal IgG1 in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05%NaN3
      Quality LevelMQ100
      Applications
      ApplicationAnti-Insulin Antibody, clone E11D7 detects level of Insulin & has been published & validated for use in ELISA, IH(P).
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      Application NotesELISA (Direct) Analysis:
      Insulin-coated plate was incubated with Anti-Insulin, clone E11D7 in 2-fold serial dilutions diluted in PBST from 5 μg/mL to 0.01 μg/mL.
      Biological Information
      ImmunogenHuman recombinant Insulin
      EpitopeInsulin
      CloneE11D7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityRecognizes insulin
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman tested. Not tested with other species, but expected to react with Mouse and Rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAfter removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • INS
      • proinsulin
      • insulin
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
      SIZE: 110 amino acids; 11981 Da
      SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
      SUBCELLULAR LOCATION: Secreted.
      DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
      SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
      Molecular Weight6 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunohistochemistry Analysis:
      20 μg/mL of this antibody detected insulin in β cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      05-1066-25UG 04054839337710

      Documentation

      Anti-Insulin Antibody, clone E11D7 (mouse monoclonal) Certificates of Analysis

      TitleLot Number
      Anti-Insulin, clone E11D7 (mouse monoclonal) - 3792009 3792009
      Anti-Insulin, clone E11D7 (mouse monoclonal) - 4059350 4059350
      Anti-Insulin, clone E11D7 (mouse monoclonal) - 4192590 4192590
      Anti-Insulin, clone E11D7 (mouse monoclonal) Monoclonal Antibody 3090199
      Anti-Insulin, clone E11D7 (mouse monoclonal) Monoclonal Antibody 3035062
      Anti-Insulin, clone E11D7 (mouse monoclonal) Monoclonal Antibody 3059617
      Anti-Insulin, clone E11D7 - 3389002 3389002