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MAB1987Z Anti-Integrin β1 Antibody, clone P4C10, azide free

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MAB1987Z
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, ICC, IP, FUNC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1987Z
      Replaces04-1109
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β1 Antibody, clone P4C10, azide free
      Alternate Names
      • CD29
      • MAB1987
      Background InformationIntegrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
      References
      Product Information
      FormatPurified
      Control
      • Human Tonsil, human skin, human lung, liver, and skeletal muscle tissues
      PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Integrin β1 Antibody, clone P4C10, azide free detects level of Integrin β1 & has been published & validated for use in ELISA, IC, IP, FUNC & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunoprecipitation
      • Affects Function
      • Western Blotting
      Application NotesInhibits attachment of UCLA-P3 cells to collagen type I (Wayner, 1991). Inhibits endodermal and keratinocytes to collagen, fibronectin and laminin. It also inhibits cell to cell adhesion. Typical dilution ranges are 1:50-1:500.

      Immunoflourescence

      Immunoblotting

      Immunoprecipitation

      ELISA

      Optimal working dilutions must be determined by end user.
      Biological Information
      CloneP4C10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with the beta-1 subunit of the integrin protein family. Appears to be human specific.Unlike many beta-1 monoclonals 4C10's epitope is not trypsin sensitive.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
      Gene Symbol
      • ITGB1
      • GPIIA
      • OTTHUMP00000063731
      • OTTHUMP00000046253
      • OTTHUMP00000063733
      • VLAB
      • OTTHUMP00000063732
      • MDF2
      • FNRB
      • CD29
      • MSK12
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.


      SIZE: 798 amino acids; 88465 Da

      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.

      TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).





      SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Molecular Weight88 KDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB1987Z 04053252616860

      Documentation

      Anti-Integrin β1 Antibody, clone P4C10, azide free SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Integrin β1 Antibody, clone P4C10, azide free Certificates of Analysis

      TitleLot Number
      Anti-Integrin 1, clone P4C10, azide free - 1940110 1940110
      Anti-Integrin 1, clone P4C10, azide free - 1987049 1987049
      Anti-Integrin 1, clone P4C10, azide free - 2026855 2026855
      Anti-Integrin 1, clone P4C10, azide free - 2090123 2090123
      Anti-Integrin 1, clone P4C10, azide free - 2325118 2325118
      Anti-Integrin 1, clone P4C10, azide free - LV1757000 LV1757000
      Anti-Integrin 1, clone P4C10, azide free - NG1894353 NG1894353
      Anti-Integrin 1, clone P4C10, azide free - NG1914009 NG1914009
      Anti-Integrin β1, clone P4C10, 2465272
      Anti-Integrin β1, clone P4C10, azide free 3075610

      References

      Reference overviewApplicationPub Med ID
      Interplay between β1-integrin and Rho signaling regulates differential scattering and motility of pancreatic cancer cells by snail and Slug proteins.
      Shields, MA; Krantz, SB; Bentrem, DJ; Dangi-Garimella, S; Munshi, HG
      The Journal of biological chemistry  287  6218-29  2012

      Show Abstract
      22232555 22232555
      Perisynaptic chondroitin sulfate proteoglycans restrict structural plasticity in an integrin-dependent manner.
      Orlando, C; Ster, J; Gerber, U; Fawcett, JW; Raineteau, O
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  18009-17, 18017a  2012

      Show Abstract
      23238717 23238717
      Integrin dynamics produce a delayed stage of long-term potentiation and memory consolidation.
      Babayan, AH; Kramár, EA; Barrett, RM; Jafari, M; Häettig, J; Chen, LY; Rex, CS; Lauterborn, JC; Wood, MA; Gall, CM; Lynch, G
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  12854-61  2012

      Show Abstract
      22973009 22973009
      Differential monocyte/macrophage interleukin-1? production due to biomaterial topography requires the ?2 integrin signaling pathway.
      Angela M B Collie,Paige C S Bota,Rachel E Johns,Ronald V Maier,Patrick S Stayton
      Journal of biomedical materials research. Part A  96  2011

      Show Abstract
      21105164 21105164
      Spinal matrix metalloproteinase-9 contributes to physical dependence on morphine in mice.
      Liu, WT; Han, Y; Liu, YP; Song, AA; Barnes, B; Song, XJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience  30  7613-23  2010

      Show Abstract
      20519536 20519536
      Targeting of DC-SIGN on human dendritic cells by minor fimbriated Porphyromonas gingivalis strains elicits a distinct effector T cell response.
      Zeituni, AE; Jotwani, R; Carrion, J; Cutler, CW
      Journal of immunology (Baltimore, Md. : 1950)  183  5694-704  2009

      Show Abstract Full Text Article
      19828628 19828628
      Integrin regulation of cytoplasmic calcium in excitatory neurons depends upon glutamate receptors and release from intracellular stores.
      Lin, CY; Hilgenberg, LG; Smith, MA; Lynch, G; Gall, CM
      Molecular and cellular neurosciences  37  770-80  2008

      Show Abstract Full Text Article
      Western Blotting18289871 18289871
      Induction of cell adhesion by galectin-8 and its target molecules in Jurkat T-cells.
      Hitomi Yamamoto, Nozomu Nishi, Hiroki Shoji, Aiko Itoh, Liang-Hao Lu, Mitsuomi Hirashima, Takanori Nakamura
      Journal of biochemistry  143  311-24  2008

      Show Abstract
      18024965 18024965
      Roles of phosphatidylinositol 3-kinase and NF-kappaB in human cytomegalovirus-mediated monocyte diapedesis and adhesion: strategy for viral persistence.
      Smith, MS; Bivins-Smith, ER; Tilley, AM; Bentz, GL; Chan, G; Minard, J; Yurochko, AD
      Journal of virology  81  7683-94  2007

      Show Abstract Full Text Article
      17507481 17507481
      Integrins control dendritic spine plasticity in hippocampal neurons through NMDA receptor and Ca2+/calmodulin-dependent protein kinase II-mediated actin reorganization.
      Shi, Y; Ethell, IM
      The Journal of neuroscience : the official journal of the Society for Neuroscience  26  1813-22  2006

      Show Abstract
      16467530 16467530

      Data Sheet

      Title
      MOUSE ANTI-HUMAN INTEGRIN BETA1 MONOCLONAL ANTIBODY

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      Catalog Number Description  
      MAB1987 Anti-Integrin β1 Antibody, clone P4C10 Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies