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MABT409 Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody

This Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody is validated for use in Western Blotting and Immunocytochemistry and Activity Assay and Immunoprecipitation and Immunohistochemistry for the detection of Integrin Beta1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ITGB1, FNRB, MDF2, MSK12 UniProt Number: P05556
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MABT409
100 µg  
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      Overview

      Replacement Information

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, Po, Sh, Eq, Ca, Rhesus MacaqueWB, ICC, ACT, IP, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT409
      DescriptionAnti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody
      Alternate Names
      • Integrin beta-1
      • Fibronectin receptor subunit beta
      • Glycoprotein Iia
      • GPIIA
      • VLA-4 subunit beta
      • CD29
      Background InformationIntegrin beta-1, also known as Fibronectin receptor subunit beta (FNRB), and Glycoprotein IIa (GPIIA), is a transmembrane glycoprotein that forms noncovalent complexes with various alpha integrin subunits to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Interactions between integrins and extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. Phosphorylation of threonines 788 and 789 on integrin beta1 receptor may play a key role in cell-cycle dependent regulation.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG1κ in buffer containing PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody is validated for use in Western Blotting and Immunocytochemistry and Activity Assay and Immunoprecipitation and Immunohistochemistry for the detection of Integrin Beta1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Activity Assay
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesActivity Assay Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in this functionally blocking antibody abrogated AGS cell attachment to fibronectin while causing only a slight inhibition of cell attachment to vitronectin (Hutton, M., et. al. (2010). Infection and Immunity. 78(11):4523-4531).
      Immunohistochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in floating villi tissue (Damsky, C. H., et al. (1992). Journal of Clinical Investigation. 89:210-222).
      Immunohistochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in CTB aggregates that have invaded matrigel in vitro (Damsky, C. H., et al. (1994). Development. 120:3657-3666).
      Immunocytochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in AGS cells (Hutton, M., et. al. (2010). Infection and Immunity. 78(11):4523-4531).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin Beta1, clone AIIB2 (Azide Free) in NP-40 cell lysate (Werb, Z., et al. (1989). JCB. 109:877-889).
      Biological Information
      ImmunogenWhole cells corresponding to human Integrin Beta1, clone AIIB2 (Azide Free).
      CloneAIIB2
      Concentration1 mg/mL
      HostRat
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Bovine
      • Pig
      • Sheep
      • Horse
      • Canine
      • Rhesus Macaque
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITGB1
      • FNRB
      • MDF2
      • MSK12
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~110 kDa observed. The calculated molecular weight of this protein is 88 kDa, but can be observed at ~110 kDa due to high glycosylation.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HUVEC cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Integrin Beta1, clone AIIB2 (Azide Free) in 10 µg of HUVEC cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABT409 04053252993138

      Documentation

      Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody Certificates of Analysis

      TitleLot Number
      Anti-Integrin Beta1, clone AIIB2 (Azide -2545775 2545775
      Anti-Integrin Beta1, clone AIIB2 (Azide -2548075 2548075
      Anti-Integrin Beta1, clone AIIB2 (Azide -2550072 2550072
      Anti-Integrin Beta1, clone AIIB2 (Azide -2665829 2665829
      Anti-Integrin Beta1, clone AIIB2 (Azide -2794967 2794967
      Anti-Integrin Beta1, clone AIIB2 (Azide Free) 3079120
      Anti-Integrin Beta1, clone AIIB2 (Azide Free) - Q2452912 Q2452912
      Anti-Integrin Beta1, clone AIIB2 (Azide Free) - 2888556 2888556
      Anti-Integrin Beta1, clone AIIB2 (Azide Free) - 3194631 3194631
      Anti-Integrin Beta1, clone AIIB2 (Azide Free) - 3232379 3232379
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