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MABT1536-25UG Anti-KIF2 Antibody, clone 2B12.C1

Anti-KIF2, clone 2B12.C1, Cat. No. MABT1536, is a mouse monoclonal antibody that detects Kinesin-like protein KIF2A and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

MABT1536-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1536-25UG
      DescriptionAnti-KIF2 Antibody, clone 2B12.C1
      Alternate Names
      • Kinesin-2
      • Kinesin-like protein KIF2A
      Background InformationKinesin-like protein KIF2A (UniProt: P28740; also known as Kinesin-2, KIF2A) is encoded by the Kif2a (also known as Kif2, Kns2) gene (Gene ID: 16563) in murine species. KIF2A is a member of the Kinesin-13 family that acts as a central regulator of the microtubule cytoskeleton in neurons. Proteins in this family include KIF2A, KIF2B, KIF2C/ MCAK, and KIF24. KIF2A acts as an ATP-dependent microtubule destabilizer and regulates cell migration, axon elongation, and pruning in the developing nervous system. KIF2A is highly phosphorylated in the brain and has many phosphorylation sites (Thr186/Thr187; Thr439/Ser440/Ser441, Ser470 and Ser488) that are shown to be specific targets of p21-activated kinase 1 (PAK1). Phosphorylation is reported to transform normal KIF2A into accelerating KIF2A (A-KIF2A) or braking KIF2A (B-KIF2A) that regulate microtubule dynamics and neuron morphology in response to extracellular signals. It has been shown that brain-derived neurotrophic factor (BDNF) stimulates PAK1 and CDK5 kinases, which can reduce microtubule polymerization activity of KIF2A via B-type phosphorylation. This results in enhanced outgrowth of neural processes. On the other hand, lysophosphatidic acid (LPA) can induce ROCK2 kinase activity that suppresses neurite outgrowth from round cells via A-type phosphorylation. (Ref.: Ogawa, T., and Hirokawa, N. (2015). Cell Reports 12(11); 1774-1788; Homma, N., et al. (2018). eLife.7:e30935).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-KIF2, clone 2B12.C1, Cat. No. MABT1536, is a mouse monoclonal antibody that detects Kinesin-like protein KIF2A and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected KIF2 in mouse testis tissue sections.

      Western Blotting Analysis: 1 µg/mL from a representative lot detected KIF2 in mouse brain tissue lysate.
      Biological Information
      ImmunogenT7-tagged full-length mouse recombinant Kinesin-like protein KIF2A.
      Clone2B12.C1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2B12.C1 is a mouse monoclonal antibody that detects Kinesin-like protein KIF2A in murine species.
      IsotypeIgGκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Kif2a
      • Kif2
      • Kns2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight79.75 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse brain tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected KIF2 in mouse brain tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABT1536-25UG 04054839007194