Millipore Sigma Vibrant Logo

MABT401 Anti-Lubricin Antibody/Proteoglycan 4, clone 9G3

View Products on Sigmaaldrich.com
MABT401
100 µg  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, B, Ca, Gt, Gp, PorcineWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT401
      DescriptionAnti-Lubricin Antibody/Proteoglycan 4, clone 9G3
      Alternate Names
      • Proteoglycan 4
      • Lubricin
      • Megakaryocyte-stimulating factor
      • Superficial zone proteoglycan
      • Proteoglycan 4 C-terminal part
      Background InformationThe protein called Lubricin/Proteoglycan 4 is an important secreted protein in the joint critical for joint function and thus is also a focus of research in joint diseases as well. Lubricin/Proteoglycan 4 plays a role in boundary lubrication and prevents the deposition of cartilage from the synovial fluid by controlling the synovial growth of the synovial cells and inhibiting the adhesion of the cells to the cartilage surface. During development, Lubricin/Proteoglycan 4 also acts as a growth factor for hematopoietic and endothelial cell lineages, and Lubricin/Proteoglycan 4 appears important for proper barrier and lubrication development in tissues like the eye. Lubricin/Proteoglycan 4 is highly expressed in joints and near joint boundaries but can also be found in the brain, heart, lung, kidney and other tissues. Lubricin/Proteoglycan 4 has several isoforms that differ in their cell and tissue expression. Isoform C and D are the most widely expressed forms. Finally because of its effects on surfaces, Lubricin/Proteoglycan 4 is being examined in tissue engineering and artificial cartilage development as a treatment for joint diseases.
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Lubricin Antibody/Proteoglycan 4, clone 9G3 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Lubricin/Proteoglycan 4.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lubricin/Proteoglycan 4 in human liver and human heart tissue.
      Immunohistochemistry Analysis: A representative lot detected accelerated lubricin loss at the superficial zone of articular cartilage from rats upon forced joint exercise following acute knee injury by anterior cruciate ligament (ACL) transection (ACLT) (Elsaid, K.A, et al. (2012). Osteoarthritis Cartilage. 20(8):940-948).
      Immunohistochemistry Analysis: A representative lot detected Lubricin/Proteoglycan 4 in rat arterial cartilage tissue (Jay, G.D, et al. (2012). Arthritis Rheumatism. 64(4):1162-1171).
      Western Blotting Analysis: A representative lot detected purified human synovial lubricin, as well as lubricin in synovial fluid recovered from patients with osteoarthritis (OA), camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), or from bovine, porcine, goat, dog, rat, and guinea pig (Ai, M., et al. (2015). PLoS One. 10(2):e0116237).
      Biological Information
      ImmunogenRecombinant protein corresponding to human Lubricin/Proteoglycan 4.
      Clone9G3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      • Bovine
      • Canine
      • Goat
      • Guinea Pig
      • Porcine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRG4
      • MSF
      • SZP
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~220 kDa observed. The calculated molecular weight of this protein is 151 kDa with many isoforms observed around 145 kDa and it can run higher due to glycosylation.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Lubricin purified protein.

      Western Blotting Analysis: 0.02 µg/mL of this antibody detected 10 µg of purified Lubricin/Proteoglycan 4 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalog Number GTIN
      MABT401 04055977112061