Anti-MLN64/STARD3, clone 1STAR-2G5

Clone 1STAR‐2G5 is a mouse monoclonal antibody that detects human StAR-related lipid transfer protein 3 (STARD3). It targets an epitope within the START domain.

Anti-MLN64/STARD3, clone 1STAR-2G5, Cat. No. MABS1945, is a mouse monoclonal antibody that detects human StAR-related lipid transfer protein 3 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Immunocytochemistry Immunoprecipitation Western Blotting

Immunoprecipitation Analysis: A representative immunoprecipitated MLN64/STARD3 in Immunoprecipitation applications (Di Mattia, T., et. al. (2018). EMBO Rep. 19(7); e45453).

Western Blotting Analysis: A representative lot detected MLN64/STARD3 in Western Blotting applications (Di Mattia, T., et. al. (2018). EMBO Rep. 19(7); e45453).

His-tagged recombinant fragment corresponding to 114 amino acids from the START domain of human StAR-related lipid transfer protein 3.

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

StAR-related lipid transfer protein 3 (UniProt: Q14849; also known as Metastatic lymph node gene 64 protein, MLN 64, Protein CAB1, START domain-containing protein 3, StARD3) is encoded by the STARD3 (also known as CAB1, MLN64) gene (Gene ID: 10948) in human. StARD3 is a multi-pass, homodimeric membrane protein that localizes to contact sites between the endoplasmic reticulum and late endosomes. It is a sterol-binding protein that mediates cholesterol transport from the endoplasmic reticulum to endosomes and creates contact site between the endoplasmic reticulum and late endosomes. It acts as a lipid transfer protein that redirects sterol to the endosome at the expense of the cell membrane and favors membrane formation inside endosomes. It may also mediate cholesterol transport between other membranes, such as the mitochondrial membrane or cell membrane. StARD3 contains a START domain (aa 230-443) that mediates lipid-transfer between membranes and has a hydrophobic cavity that can accommodate one lipid molecule, thereby serving as a ′hydrophobic bridge′ across the aqueous gap between donor and acceptor organelle membranes. It also has a MENTAL domain (aa 46-217) that anchors the protein in endosome membranes and exposes the START domain in the cytosol. StARD3 is shown to interact with VAMP-associated proteins A and B (VAPA and VAPB) via its FFAT (two phenylalanines in an acidic tract (FFAT) motif (aa 207-212). (Ref.: Di Mattia, T., et al. (2018). EMBO Rep. 19(7); e45453; Alpy, F., et al. (2013). J. Cell Sci. 126(23); 5500-5012).

Evaluated by Immunocytochemistry in SK-BR-3 cell line.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected MLN64/STARD3 in SK-BR-3 cells.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.