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MAB4100 Anti-MRP1 Antibody, clone QCRL-1

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MAB4100
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, WB, ICC, IHC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4100
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MRP1 Antibody, clone QCRL-1
      Alternate Names
      • Multidrug Resistance Protein-1
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified immunoglobulin. Liquid in PBS with 0.1% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P).
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996).

      Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).

      Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).

      Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).

      Immunoprecipitation (Hipfner, et al., 1994; 1996).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenNon-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999).
      CloneQCRL-1
      HostMouse
      SpecificityReacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane

      phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines

      and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,

      MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternative splicing by exon deletion results in several splice variants but maintains the original open reading frame in all forms.
      Gene Symbol
      • ABCC1
      • 4transporter)
      • ABC29
      • GS-X
      • ABCC
      • MRP1
      • DKFZp781G125
      • MRP
      • DKFZp686N04233
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P33527 # May participate directly in the active transport of drugs into subcellular organelles or influence drug distribution indirectly. Confers resistance to anticancer drugs. Transports LTC4. May protect milk against xenobiotics (By similarity).
      SIZE: 1531 amino acids; 171561 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Lung, testis and peripheral blood mononuclear cells.
      SIMILARITY: SwissProt: P33527 ## Belongs to the ABC transporter family. Conjugate transporter (TC 3.A.1.208) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8ºC for up to 3 months. For longer term storage, aliquot and freeze at -20ºC.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB4100 04053252666551

      Documentation

      Anti-MRP1 Antibody, clone QCRL-1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MRP1 Antibody, clone QCRL-1 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-MRP1 [MULTIDRUG RESISTANCE PROTEIN-1] MONOCLONAL ANTIBODY - 2137000 2137000
      MOUSE ANTI-MRP1 [MULTIDRUG RESISTANCE PROTEIN-1] MONOCLONAL ANTIBODY - 2387838 2387838
      MOUSE ANTI-MRP1 [MULTIDRUG RESISTANCE PROTEIN-1] - 2510328 2510328
      MOUSE ANTI-MRP1 [MULTIDRUG RESISTANCE PROTEIN-1] - 3217064 3217064
      MOUSE ANTI-MRP1 [MULTIDRUG RESISTANCE PROTEIN-1] - 3501008 3501008
      MOUSE ANTI-MRP1 [MULTIDRUG RESISTANCE PROTEIN-1] - 3793076 3793076
      MOUSE ANTI-MRP1 [MULTIDRUG RESISTANCE PROTEIN-1] - 3842829 3842829
      MOUSE ANTI-MRP1 [MULTIDRUG RESISTANCE PROTEIN-1] - 3936241 3936241
      MOUSE ANTI-MRP1 [MULTIDRUG RESISTANCE PROTEIN-1] - 4187209 4187209
      MOUSE ANTI-MRP1 [MULTIDRUG RESISTANCE PROTEIN-1] -2651931 2651931

      References

      Reference overviewPub Med ID
      A rapid and quantitative coat protein complex II vesicle formation assay using luciferase reporters.
      J Chris Fromme,Jinoh Kim
      Analytical biochemistry  421  2012

      Show Abstract
      22244805 22244805
      Ligand-independent higher-order multimerization of CXCR4, a G-protein-coupled chemokine receptor involved in targeted metastasis.
      Makiko Hamatake, Toru Aoki, Yuko Futahashi, Emiko Urano, Naoki Yamamoto, Jun Komano, Makiko Hamatake, Toru Aoki, Yuko Futahashi, Emiko Urano, Naoki Yamamoto, Jun Komano
      Cancer science  100  95-102  2009

      Show Abstract
      19018754 19018754
      Cells resistant to HTI-286 do not overexpress P-glycoprotein but have reduced drug accumulation and a point mutation in alpha-tubulin.
      Frank Loganzo, Malathi Hari, Tami Annable, Xingzhi Tan, Daniel B Morilla, Sylvia Musto, Arie Zask, Joshua Kaplan, Albert A Minnick, Michael K May, Semiramis Ayral-Kaloustian, Marianne S Poruchynsky, Tito Fojo, Lee M Greenberger
      Molecular cancer therapeutics  3  1319-27  2004

      Show Abstract
      15486199 15486199
      Efficacy of MGI 114 (HMAF) against the MRP+ metastatic MV522 lung carcinoma xenograft.
      M J Kelner, T C McMorris, L A Estes, M Y Oval, R J Rojas, J R Lynn, K A Lanham, K M Samson
      Anti-cancer drugs  11  217-24  2000

      Show Abstract
      10831281 10831281
      Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line.
      Cole, S P, et al.
      Science, 258: 1650-4 (1992)  1992

      Show Abstract
      1360704 1360704

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies