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05-724-25UG Anti-Myc Tag Antibody, clone 4A6 (mouse monoclonal IgG1)

This Anti-Myc Tag Antibody, clone 4A6 is validated for use in ChIP, IC, IF, IP, WB for the detection of Myc Tag. This antibody has been cited in more than 25 published papers.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epitope Tags & General Use Research Sub-Category: Epitope Tags Gene Symbol: MYC, C-MYC, c-Myc UniProt Number: P01106
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05-724-25UG
25 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IF, IP, WB, ChIP-seqMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-724-25UG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Myc Tag Antibody, clone 4A6 (mouse monoclonal IgG1)
      Background InformationEpitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
      The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
      References
      Product Information
      FormatPurified
      Control
      • Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
      PresentationProtein G Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Myc Tag Antibody, clone 4A6 is validated for use in ChIP, IC, IF, IP, WB for the detection of Myc Tag. This antibody has been cited in more than 25 published papers.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      • ChIP-seq
      Biological Information
      Immunogenpeptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc
      Clone4A6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityRecognizes recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts; also recognizes human Myc.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
      Gene Symbol
      • MYC
      • C-MYC
      • c-Myc
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
      SIZE: 439 amino acids; 48804 Da
      SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Phosphorylated by PRKDC.
      DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular WeightPredicted MW for human Myc: 49 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag, clone 9E10 (Catalogue No. CBL430)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2 to 8°C for 1 year from date of shipment.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      05-724-25UG 04054839337659

      Documentation

      Anti-Myc Tag Antibody, clone 4A6 (mouse monoclonal IgG1) Certificates of Analysis

      TitleLot Number
      Anti-Myc Tag, clone 4A6 (mouse monoclonal IgG1) - 3208183 3208183
      Anti-Myc Tag, clone 4A6 (mouse monoclonal IgG1) - 3733739 3733739
      Anti-Myc Tag, clone 4A6 (mouse monoclonal IgG1) - 4022126 4022126
      Anti-Myc Tag, clone 4A6 (mouse monoclonal IgG1) - 4071245 4071245
      Anti-Myc Tag, clone 4A6 (mouse monoclonal IgG1) - 4131108 4131108
      Anti-Myc Tag, clone 4A6 (mouse monoclonal IgG1) - 4169237 4169237
      Anti-Myc Tag, clone 4A6 - 3249914 3249914
      Anti-Myc Tag, clone 4A6 - 3328186 3328186
      Anti-Myc Tag, clone 4A6 - 3519282 3519282