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ABN45 Anti-Myelin Regulatory Factor (MRF) Antibody

This Anti-Myelin Regulatory Factor (MRF) Antibody is validated for use in western blotting & IHC for the detection of Myelin Regulatory Factor (MRF).

Anti-Myelin Regulatory Factor (MRF) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: MYRF, C11orf9, KIAA0954, MRF UniProt Number: Q9Y2G1
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ABN45
100 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN45
      DescriptionAnti-Myelin Regulatory Factor (MRF) Antibody
      Alternate Names
      • Myelin regulatory factor
      • Myelin gene regulatory factor
      • Myelin regulatory factor, N-terminal
      • Myelin regulatory factor, C-terminal
      Background InformationMyelin Regulatory Factor (MRF), also known as Myelin regulatory factor, MYRF, and encoded by the gene MYRF/C11orf9/KIAA0954/MRF, is an interesting protein that gets cleaved into two chains. The N terminal component specifically activates transcription of myelin gene in the CNS oligodendrocytes and pre-oligodendrocytes. The C-terminal portion, which is remains membrane bound, appears to play no role in gene induction but its complete role is still being discovered. The N-terminal portion of MRF activates MBP, MOG, MAG, and PLP among other genes, but it is not required for oligodendrocyte differentiation but is required for the maintenance of myelin in the adult. MRF whole factor is localized to the endoplasmic reticulum. The cleaved N terminal portion translocates to the nucleus and the C-terminal portion remains in the endoplasmic reticulum. Interestingly MRF can be found expressed outside of oligodendrocytes and oligo precursors in the brain including regions such as the lung, basal ganglion and liver.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Myelin Regulatory Factor (MRF) Antibody is validated for use in western blotting & IHC for the detection of Myelin Regulatory Factor (MRF).
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected Myelin Regulatory Factor (MRF) in 10 µg of ARPE19 cell lysate.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Myelin Regulatory Factor (MRF) in human cerebral cortex, human retina, and human cerebellum tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Myelin Regulatory Factor (MRF).
      Epitopecytoplasmic domain, N-terminal chain
      Concentration1.0 mg/mL
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Hamster, Horse, Monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MYRF
      • C11orf9
      • KIAA0954
      • MRF
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~160 kDa observed. The expected molecular weight is 125 kDa and ~160kDa when glycosylated. The protein was cleaved into a ~90 kDa N-terminal fragment from the full length of ~160 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myelin Regulatory Factor (MRF) in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABN45 04053252977527

      Documentation

      Anti-Myelin Regulatory Factor (MRF) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Myelin Regulatory Factor (MRF) Antibody Certificates of Analysis

      TitleLot Number
      Anti-Myelin Regulatory Factor (MRF) - Q2431448 Q2431448
      Anti-Myelin Regulatory Factor (MRF) - 3233121 3233121
      Anti-Myelin Regulatory Factor (MRF) - 3277599 3277599
      Anti-Myelin Regulatory Factor (MRF) - 3389043 3389043
      Anti-Myelin Regulatory Factor (MRF) - 3587272 3587272
      Anti-Myelin Regulatory Factor (MRF) - 4180375 4180375
      Anti-Myelin Regulatory Factor (MRF) -2625370 2625370
      Anti-Myelin Regulatory Factor (MRF) -2652011 2652011
      Anti-Myelin Regulatory Factor (MRF) -2668508 2668508
      Anti-Myelin Regulatory Factor (MRF) -2770274 2770274
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