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ABE2867-25UL Anti-NR5A2 (LRH1)

Anti-NR5A2 (LRH1), Cat. No. ABE2867-25UL, is a highly specific rabbit polyclonal antibody, that targets Nuclear receptor subfamily 5 group A member 2 and has been tested in Western Blotting, Immunofluorescence and Chromatin Immunoprecipitation (ChIP).

Anti-NR5A2 (LRH1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: NR5A2, B1F, CPF, FTF UniProt Number: O00482
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ABE2867-25UL
25 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IF, ChIPRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2867-25UL
      DescriptionAnti-NR5A2 (LRH1)
      Alternate Names
      • Nuclear receptor subfamily 5 group A member 2
      • Alpha-1-fetoprotein transcription factor
      • B1-binding factor
      • hB1F
      • CYP7A promoter-binding factor
      • Hepatocytic transcription factor
      • Liver receptor homolog 1
      • LRH-1
      Background InformationNuclear receptor subfamily 5 group A member 2 (UniProt: O00482; also known as Alpha-1-fetoprotein transcription factor, B1-binding factor, hB1F, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, LRH-1) is encoded by the NR5A2 (also known as B1F, CPF, FTF) gene (Gene ID: 2494) in human. It is a member of the nuclear receptor superfamily that binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes. It contains a DNA binding region (aa 83-104) and two NR C4-type zinc finger domains (aa 86-106 and 122-146). It is abundantly expressed in pancreas, but at reduced levels in liver, and at very low levels in heart and lung. It s expression has been demonstrated in the Hep-G2 cell line. Four different isoforms of LRH-1 have been described that are generated by alternate splicing. LRH-1 is reported to be involved in the regulation of expression of transcription factors, such as Oct4, HNF1, 3, and 4, which are known to participate in embryonic development. Its presence in high levels in beta-III Tubulin+ and NeuN+ neurons of the mantle zone (MZ) in early neural tube of mice (E10.5 and E12.5) indicate its involvement in neural differentiation. (Ref.: Stergiopoulos, A., and Politis, PK (2016). Nat. Commun. DOI: 10.1038/ncomms12230; Venteclef, N et al. (2008). J. Biol. Chem. 283(7); 3694 3701).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Applications
      ApplicationAnti-NR5A2 (LRH1), Cat. No. ABE2867-25UL, is a highly specific rabbit polyclonal antibody, that targets Nuclear receptor subfamily 5 group A member 2 and has been tested in Western Blotting, Immunofluorescence and Chromatin Immunoprecipitation (ChIP).
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation Analysis: A representative lot detected NR5A2 (LRH1) in Chromatin Immunoprecipitation applications (Kaltezioti, V., et. al. (2010). PLoS Biol. 8(12):e1000565; Venteclef, N., et. al. (2010). Genes Dev. 24(4):381-95; Boulias, K., et. al. (2005). EMBO J. 24(14):2624-33; Chalkiadaki, A., et. al. (2005). Mol Cell Biol. 25(12):5095-105).

      Western Blotting Analysis: A representative lot detected NR5A2 (LRH1) in Western Blotting applications (Chalkiadaki, A., et. al. (2005). Mol Cell Biol. 25(12):5095-105; Stergiopoulos, A., et. al. (2016). Nat Commun. 7:12230).

      Immunofluorescence Analysis: A representative lot detected NR5A2 (LRH1) in Immunofluorescence YES titration paraffin sections from E10.5 (a), E12.5 (b), E14.5 (c) or E16.5 (d) mouse spinal cord (Stergiopoulos, A., et. al. (2016). Nat Commun. 7:12230).
      Biological Information
      ImmunogenHis-tagged full length recombinant human Nuclear receptor subfamily 5 group A member 2, isoform 2.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Nuclear receptor subfamily 5 group A member 2 (NR5A2) in human and murine cells.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • NR5A2
      • B1F
      • CPF
      • FTF
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight68 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.
      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NR5A2 (LRH1) in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABE2867-25UL 04054839474910