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MABS2278-25UL Anti-Oxidized phospholipid Antibody, clone EO6

Anti-Oxidized phospholipid, clone EO6, Cat. No. MABS2278, is a mouse monoclonal antibody that detects oxidized phospholipids and is tested for use in ELISA, Immunohistochemistry, Radioimmunoassay, and Western Blotting.

MABS2278-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2278-25UL
      DescriptionAnti-Oxidized phospholipid Antibody, clone EO6
      Alternate Names
      • Ox-PC
      • OX-Phospholipid
      Background InformationSeveral reactive lipid peroxidation products are generated during the oxidation of LDL, and modification of lipids and apoproteins occurs in vivo. These oxidized and/modified LDL have the ability to bind to scavenger receptors that can lead to unregulated accumulation of cholesterol in macrophages and formation of foam cells. These products can also react with lipids, apoproteins, and proteins and generate immunogenic neoepitopes and contribute to atherogenesis. The lipid peroxidation products can for adducts with free amino groups of lysines and other amino acids in apolipoproteins. Reactive oxygen species (ROS) and oxidized phospholipids are also reported to activate TRPA1 and TRPV1 cation ion channels that shape thermal and mechanical hypersensitivity in inflammation and pain. These channels are also suggested to be involved in pathological events, including rheumatoid arthritis and trigeminal neuropathic pain. Even locally injected oxidized lipids can elicit thermal and mechanical hypersensitivity and acute nociception. Clone E06/T15 is a monoclonal autoantibody that specifically binds to the head group of oxidized phosphatidylcholines in oxidized phospholipids. (Ref.: Mohammadi, M., et al. (2018). Br. J. Pharmacol. 175(14); 2940-2955; Hörkkö, S., et al. (1999). J. Clin. Invest. 103(1): 117-128; Palinski, W., et al. (1996). J. Clin. Invest. 98:800 814.).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Oxidized phospholipid, clone EO6, Cat. No. MABS2278, is a mouse monoclonal antibody that detects oxidized phospholipids and is tested for use in ELISA, Immunohistochemistry, Radioimmunoassay, and Western Blotting.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Western Blotting
      • Radioimmunoassay
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected OxPL in Western Blotting applications (Horkko, S., et al. (1999). J Clin Invest. 103(1):117-28; Leibundgut, G., et al. (2013). J Lipid Res. 54(10)2815-30).

      ELISA Analysis: A representative lot detected OxPL in ELISA applications (Horkko, S., et al. (1999). J Clin Invest. 103(1):117-28).

      Immunohistochemistry Applications: A representative lot detected Oxidized phospholipid in Immunohistochemistry applications (Yu, P., et al. (2018). Arterioscler Thromb Vasc Biol. 38(1):26-39).

      Radioimmunoassay: A representative lot detected OxPL in Radioimmunoassay applications (Horkko, S., et al. (1999). J Clin Invest. 103(1):117-28).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenB lymphocytes from the spleens of ApoE-deficient mice fused with a myeloma cell line and selected hybridomas were cloned and cells were injected intraperitoneally into mice as described by Hörkkö et al. (1999).
      EpitopeUnknown
      CloneEO6
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone E06 is a mouse monoclonal antibody that binds to oxidized phospholipids and reacts with oxidized LDL, oxidized HDL and proteins covalently modified by oxidized phospholipids.
      IsotypeIgMκ
      Species Reactivity
      • All
      Species Reactivity NoteAll.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodLigatrap
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA with Oxidized Phosphatidylcholine-BSA (OxPC-BSA)

      ELISA Analysis: A serial dilution of this antibody detected Oxidized-Phosphatidylcholine-BSA.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS2278-25UL 04065270321787