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ABS1487-25UG Anti-P2Y12 Receptor

Anti-P2Y12 Receptor, Cat. No. ABS1487, is a rabbit polyclonal antibody that detects P2Y purinoceptor 12 and has been tested for Immunohistochemistry (Paraffin) and Western Blotting.

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ABS1487-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS1487-25UG
      DescriptionAnti-P2Y12 Receptor
      Alternate Names
      • P2Y purinoceptor 12
      • ADP-glucose receptor
      • ADPG-R
      • P2T(AC)
      • P2Y(AC)
      • P2Y(cyc)
      • P2Y12 platelet ADP receptor
      • P2Y(ADP)
      • SP1999
      Background InformationP2Y purinoceptor 12 (UniProt: Q9H244; also known as P2Y12, ADP-glucose receptor, ADPG-R, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, P2Y(ADP), SP1999) is encoded by the P2RY12 (also known as HORK3) gene (Gene ID: 64805) in human. P2Y12 is a multi-pass metabotropic receptor that is highly expressed in the platelets. Lower expression levels have been observed in the brain, lung, and adrenal glands. P2Y12 serves as a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. It is not activated by UDP and UTP. It is required for normal platelet aggregation and blood coagulation. It is the predominant receptor involved in the ADP-stimulated activation of the glycoprotein IIb/IIIa receptor. Activation of the glycoprotein IIb/IIIa receptor results in enhanced platelet degranulation and thromboxane production, and prolonged platelet aggregation. P2Y12 receptor has also been reported to be expressed on microglia and is required to neuropathic pain following peripheral nerve injury. P2Y12 has seven transmembrane, four cytoplasmic, and four extracellular domains. The transmembrane helices are somewhat tilted and/or kinked. Agonist binding promotes a conformation change in the extracellular loops that leads to an inward movement of the transmembrane helices. Mutations in P2RY12 gene have been linked to bleeding disorders resulting from severe impairment of platelet response to ADP resulting in defective platelet aggregation. (Ref.: Burnstock G. (2009). Curr. Pharm. Des. 15(15);1717-35; Damman, P., et al. (2012). J. Thromb. Thrombolysis. 33(2); 143-153).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-P2Y12 Receptor, Cat. No. ABS1487, is a rabbit polyclonal antibody that detects P2Y purinoceptor 12 and has been tested for Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250-1,000 dilution from a representative lot detected P2Y12 Receptor in human cerebral cortex and human tonsil tissue sections.

      Western Blotting Analysis: 1 µg/mL from a representative lot detected P2Y12 Receptor in human platelet cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the internal region of human P2Y purinoreceptor 12.
      Epitopeextracellular domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects P2Y12 receptor. It targets an epitope with in 13 amino acids from the extracellular domain.
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteHuman, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • P2RY12
      • HORK3
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~50 kDa observed; 39.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected P2Y12 Receptor in PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS1487-25UG 04054839623509