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ABS233-AF488 Anti-PI3 Kinase p85 Antibody, N-SH2 domain Antibody, Alexa Fluor® 488 Conjugate

Anti-PI3 Kinase p85, N-SH2 domain, Alexa Fluor® 488 Conjugate, Cat. No. ABS233-AF488, detects PI3 Kinase p85 and is validated for use in immunocytochemistry.

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ABS233-AF488
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS233-AF488
      DescriptionAnti-PI3 Kinase p85 Antibody, N-SH2 domain Antibody, Alexa Fluor® 488 Conjugate
      Alternate Names
      • Phosphatidylinositol 3-kinase regulatory subunit alpha
      • PI3-kinase regulatory subunit alpha
      • PI3K regulatory subunit alpha
      • PtdIns-3-kinase regulatory subunit alpha
      • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
      • PI3-kinase subunit p85-alpha
      • PtdIns-3-kinase regulatory subunit p85-alpha
      Background InformationPhosphatidylinositol 3-kinase regulatory subunit alpha (UniProt: Q63787; also known as PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha) is encoded by the Pik3r1 (Gene ID 25513) gene in rat. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. It binds to activated (phosphorylated) protein tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. PI3 Kinase catalyzes the phosphorylation of PI(4,5)P2 (PIP2) inositol ring at the 3 position to generate PI(3,4,5)P3 (PIP3), a potent second messenger that mediates the action of several growth factors. PI3 kinase is essential for the insulin signaling and also plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), bringing p85-associated p110 proximal to its lipid substrate on the membrane. PI3 Kinase is also activated by Ras and by the beta and gamma subunits of heterotrimeric G-proteins.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified rabbit polyclonal antibody Alexa Fluor® 488 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PI3 Kinase p85, N-SH2 domain, Alexa Fluor® 488 Conjugate, Cat. No. ABS233-AF488, detects PI3 Kinase p85 and is validated for use in immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. ABS233) is also available for immunoprecipitation and Western blotting applications.
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the first SH2 domain of rat PI3 kinase regulatory subunit alpha.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody targets the first SH2 domain region common to human (isoform 1, AS53, p46, p85I), mouse, and rat (isoform p85-alpha, p55-alpha, and p50-alpha) spliced isoforms of PI3 kinase regulatory subunit alpha reported by UniProt (P27986, P26450, Q63787).
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat. Predicted to react with Bovine, Monkey, Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pik3r1
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~85/50 kDa observed. 83.47/53.49/49.97/84.47 kDa (human isoform 1/AS53/p46/p85I; UniProt P27986), 83.39 kDa (mouse; UniProt P26450), 83.40/53.39/50.00 kDa (rat p85-alpha/p55-alpha/p50-alpha isoforms; UniProt Q63787).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

      Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PI3 kinase p85 subunit in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HepG2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS233-AF488 04054839134357

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