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MABC544 Anti-PLK-4 Antibody, clone 6H5

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MABC544
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC544
      DescriptionAnti-PLK-4 Antibody, clone 6H5
      Alternate Names
      • Serine/threonine-protein kinase PLK4
      • Polo-like kinase 4
      • PLK-4
      • Serine/threonine-protein kinase 18
      • Serine/threonine-protein kinase Sak
      Background InformationSerine/threonine-protein kinase PLK4 (PLK-4) is a member of the protein kinase superfamily and plays an essential role in centriole duplication. PLK-4 triggers procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins. It may be involved in tumorigenesis since centrosome aberrations are frequently observed in tumors. PLK-4 may also be involved in trophoblast differentiation by phosphorylating HAND1, leading to the disruption between HAND1 and MDFIC and then activating HAND1.
      References
      Product Information
      FormatPurified
      Control
      • U2Os cell lysate
      PresentationPurified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Polo Like Kinase (PLK) using this mouse monoclonal antibody, Anti-PLK-4 Antibody, clone 6H5 validated for use in western blotting, ICC & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: A representative lot detected PLK-4 in U20S cell lysates transfected with control and Cep152 siRNA, but not U2OS cell lysates transfected with PLK4 siRNA. (Cizmecioglu, O. et al. (2010). J Cell Biol. 191(4):731-739).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated PLK-4 in HeLa cell lysates cotransfected with Flag-tagged Gcp6 and Myc-tagged Plk-4 (Bahtz, R. et al. (2012). J Cell Sci. 125 (Pt 2): 486-496).
      Biological Information
      ImmunogenLinear peptide corresponding to Human PLK-4.
      Clone6H5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG3κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PLK4
      • SAK
      • STK18
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight109 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U2Os cell lysate.

      Western Blotting Analysis: 4.0 µg/mL of this antibody detected PLK-4 in 10 µg of U2Os cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABC544 04053252915802

      Documentation

      Anti-PLK-4 Antibody, clone 6H5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PLK-4 Antibody, clone 6H5 Certificates of Analysis

      TitleLot Number
      Anti-PLK-4, clone 6H5 - 3221099 3221099
      Anti-PLK-4, clone 6H5 - 3382301 3382301
      Anti-PLK-4, clone 6H5 - 3560117 3560117
      Anti-PLK-4, clone 6H5 - 3614216 3614216
      Anti-PLK-4, clone 6H5 - 3751200 3751200
      Anti-PLK-4, clone 6H5 - 3870649 3870649
      Anti-PLK-4, clone 6H5 - 3984070 3984070
      Anti-PLK-4, clone 6H5 - 4057976 4057976
      Anti-PLK-4, clone 6H5 - 4065785 4065785
      Anti-PLK-4, clone 6H5 - 4128265 4128265

      References

      Reference overviewPub Med ID
      STIL binding to Polo-box 3 of PLK4 regulates centriole duplication.
      Arquint, C; Gabryjonczyk, AM; Imseng, S; Böhm, R; Sauer, E; Hiller, S; Nigg, EA; Maier, T
      eLife  4  2015

      Show Abstract
      26188084 26188084
      Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole.
      Ohta, M; Ashikawa, T; Nozaki, Y; Kozuka-Hata, H; Goto, H; Inagaki, M; Oyama, M; Kitagawa, D
      Nature communications  5  5267  2014

      Show Abstract
      25342035 25342035
      GCP6 is a substrate of Plk4 and required for centriole duplication.
      Bahtz, Ramona, et al.
      J. Cell. Sci., 125: 486-96 (2012)  2012

      Show Abstract
      22302995 22302995
      Cep152 acts as a scaffold for recruitment of Plk4 and CPAP to the centrosome.
      Cizmecioglu, Onur, et al.
      J. Cell Biol., 191: 731-9 (2010)  2010

      Show Abstract
      21059844 21059844

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies