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MABS197 Anti-PTP1B Antibody, clone FG6-1G

Anti-PTP1B Antibody, clone FG6-1G is an antibody against PTP1B for use in Western Blotting, Immunoprecipitation.

Anti-PTP1B Antibody, clone FG6-1G MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: PTPN1, PTP1B UniProt Number: P18031
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MABS197
100 µg  
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      Overview

      Replacement Information

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS197
      Replaces04-1140
      DescriptionAnti-PTP1B Antibody, clone FG6-1G
      Alternate Names
      • Tyrosine-protein phosphatase non-receptor type 1
      • Protein-tyrosine phosphatase 1B
      • PTP-1B
      Background InformationPTP1B is the prototypic member of the protein tyrosine phosphatase (PTP) family, which comprises at least 37 proteins. The family is characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. The PTPs have high substrate specificity for phosphotyrosyl proteins. At the primary sequence level, PTPs share little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. PTP1B is a negative regulator of insulin and leptin signal transduction and may be a potential therapeutic target for diabetes and obesity. Insulin also influences the expression of splice variants of PTP1B.
      References
      Product Information
      FormatPurified
      Control
      • TF-1 cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PTP1B Antibody, clone FG6-1G is an antibody against PTP1B for use in Western Blotting, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blot Analysis: A representative lot was used to detect PTP1B in Jurkat cell lysate.

      Immunoprecipitation Analysis: A representative lot from an independent laboratory detected PTP1B in A431 cell lysate (Gulati, P. et al. (2004). EMBO Rep. 5(8):812-817.).
      Biological Information
      ImmunogenRecombinant protein corresponding to the catalytic domain of human PTP1B.
      EpitopeCatalytic domain
      CloneFG6-1G
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the catalytic domain of PTP1B.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
      Gene Symbol
      • PTPN1
      • PTP1B
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion.

      CATALYTIC ACTIVITY: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

      SUBUNIT STRUCTURE: Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function.

      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note: Interacts with EPHA3 at the cell membrane.

      POST-TRANSLATIONAL MODIFICATION: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.

      SEQUENCE SIMILARITIES: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. Contains 1 tyrosine-protein phosphatase domain.
      Molecular Weight~50 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in TF-1 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected PTP1B in 10 µg of TF-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS197 04053252689826

      Documentation

      Anti-PTP1B Antibody, clone FG6-1G SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PTP1B Antibody, clone FG6-1G Certificates of Analysis

      TitleLot Number
      Anti-PTP1B, clone FG6-1G 2479551
      Anti-PTP1B, clone FG6-1G - 2386525 2386525
      Anti-PTP1B, clone FG6-1G - 2432520 2432520
      Anti-PTP1B, clone FG6-1G - 2376686 2376686
      Anti-PTP1B, clone FG6-1G - 3167072 3167072
      Anti-PTP1B, clone FG6-1G - 3387625 3387625
      Anti-PTP1B, clone FG6-1G - 3585812 3585812
      Anti-PTP1B, clone FG6-1G - 3720623 3720623
      Anti-PTP1B, clone FG6-1G - 3901423 3901423
      Anti-PTP1B, clone FG6-1G - 4080485 4080485
      Show more

      References

      Reference overviewPub Med ID
      UVA inactivates protein tyrosine phosphatases by calpain-mediated degradation.
      Gulati, Pawan, et al.
      EMBO Rep., 5: 812-7 (2004)  2004

      Show Abstract       		15247926 15247926

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies