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OP40 Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10)

This Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of Pan-Ras (Ab-3).

Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP40
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Pricing & Availability

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OP40-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~21 kDa H-, K-, and N-Ras proteins in SW480 and Y1 cells and breast carcinoma tissue.
      Catalogue NumberOP40
      Brand Family Calbiochem®
      Application Data
      Detection of human Ras by immunoblotting: Sample: Whole cell lysate (56 μg) from MCF-7 cells. Primary antibody: Anti-Pan-Ras (Ab-3) Mouse mAb (RAS10) (Cat. No. OP40) (0.3 μg/ml). Detection: chemiluminescence.
      References
      ReferencesRodenhuis, S. et al., 1992. Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al. 1992. Science 256, 102.
      Hamer, P.J., et al. 1990. Hybridoma 9, 573.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M. E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Positive controlSW480 or Y1 cells or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone, Immunoblotting, Immunoprecipitation Hamer, P.J., et al. 1990. Hybridoma 9, 573.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Paraffin Sections
      Application NotesFlow Cytometry (2.5 µg/ml)
      Frozen Sections (2.5 µg/ml)
      Immunoblotting (0.1-3 µg/ml, chemiluminescent and colorimetric, see application references)
      Immunofluorescence (2.5 µg/ml, see application references)
      Immunoprecipitation (1 µg/sample)
      Paraffin Sections (2.5 µg/ml, saponin pre-treatment required)
      Application CommentsThe level of expression of p21ras is variable in different tissues. For this reason we recommend a concentration step prior to immunoblot analysis to obtain optimal results. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant human p21 Ras
      ImmunogenHuman
      CloneRAS 10
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      OP40-100UG 07790788054458

      Documentation

      Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) Certificates of Analysis

      TitleLot Number
      OP40

      References

      Reference overview
      Rodenhuis, S. et al., 1992. Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al. 1992. Science 256, 102.
      Hamer, P.J., et al. 1990. Hybridoma 9, 573.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M. E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.

      Citations

      Title
    • Marie Dance, et al. (2006) The adaptor protein gab1 couples the stimulation of vascular endothelial growth factor receptor-2 to the activation of phosphoinositide 3-kinase. Journal of Biological Chemistry 281, 23285-23295.
    • Clair C. Sharpe, et al. (1999) Evidence of a Role for Ki-RAS in the Stimulated Proliferation of Renal Fibroblasts. Journal of the American Society of Nephrology 10, 1186-1192.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-September-2007 JSW
      ApplicationFlow Cytometry (2.5 µg/ml)
      Frozen Sections (2.5 µg/ml)
      Immunoblotting (0.1-3 µg/ml, chemiluminescent and colorimetric, see application references)
      Immunofluorescence (2.5 µg/ml, see application references)
      Immunoprecipitation (1 µg/sample)
      Paraffin Sections (2.5 µg/ml, saponin pre-treatment required)
      Application Data
      Detection of human Ras by immunoblotting: Sample: Whole cell lysate (56 μg) from MCF-7 cells. Primary antibody: Anti-Pan-Ras (Ab-3) Mouse mAb (RAS10) (Cat. No. OP40) (0.3 μg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 myeloma cells (see application references). Recognizes the ~21 kDa Ras protein.
      BackgroundThe human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras, sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant human p21 Ras
      CloneRAS 10
      IsotypeIgG2a
      Specieshuman, mouse, rat
      Positive controlSW480 or Y1 cells or breast carcinoma tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe level of expression of p21ras is variable in different tissues. For this reason we recommend a concentration step prior to immunoblot analysis to obtain optimal results. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesRodenhuis, S. et al., 1992. Cancer Res. (Suppl) 52, 2665s.
      Sidransky, D., et al. 1992. Science 256, 102.
      Hamer, P.J., et al. 1990. Hybridoma 9, 573.
      Bos, J.L. 1989. Cancer Res. 49, 4682.
      Furth, M. E., et al. 1987. Oncogene 1, 47.
      Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Citation
    • Marie Dance, et al. (2006) The adaptor protein gab1 couples the stimulation of vascular endothelial growth factor receptor-2 to the activation of phosphoinositide 3-kinase. Journal of Biological Chemistry 281, 23285-23295.
    • Clair C. Sharpe, et al. (1999) Evidence of a Role for Ki-RAS in the Stimulated Proliferation of Renal Fibroblasts. Journal of the American Society of Nephrology 10, 1186-1192.
      		•
      Application referencesOriginal Clone, Immunoblotting, Immunoprecipitation Hamer, P.J., et al. 1990. Hybridoma 9, 573.