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AB2994 Anti-Phospho-Bid (Ser65) Antibody

Anti-Phospho-Bid (Ser65) Antibody detects level of Phospho-Bid (Ser65) & has been published & validated for use in WB.

Anti-Phospho-Bid (Ser65) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: FP497, MGC15319, MGC42355, OTTHUMP00000196197 UniProt Number: P55957-2
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AB2994
100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB2994
      DescriptionAnti-Phospho-Bid (Ser65) Antibody
      Alternate Names
      • BH3-interacting domain death agonist
      • p22 BID
      • BID
      Background InformationBid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Western Blot:: Jurkat cell lysate
      PresentationPurified in PBS with 0.09% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Phospho-Bid (Ser65) Antibody detects level of Phospho-Bid (Ser65) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      EpitopeSer65
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody reacts with the 22 kDa phospho-Bid-S65 protein.
      Species Reactivity
      • Human
      Species Reactivity NoteReacts with human. Other species have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq]
      Gene Symbol
      • FP497
      • MGC15319
      • MGC42355
      • OTTHUMP00000196197
      Modifications
      • Phosphorylation
      Purification MethodAntigen Affinity Purified
      UniProt Number
      UniProt SummaryFUNCTION:The major proteolytic product p15 BID allows the release of cytochrome c By similarity. Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

      SUBUNIT STRUCTURE: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2 By similarity.

      SUBCELLULAR LOCATION:CytoplasmBy similarity. Mitochondrion membraneBy similarity. Note= When uncleaved, it is predominantly cytoplasmic.

      BH3-interacting domain death agonist p15: Mitochondrion membraneBy similarity. Note= Translocates to mitochondria as an integral membrane protein By similarity.

      BH3-interacting domain death agonist p13: Mitochondrion membraneBy similarity. Note= Associated with the mitochondrial membrane By similarity.

      Isoform 1: Cytoplasm. Ref.3

      Isoform 3: Cytoplasm. Ref.3

      Isoform 2: Mitochondrion membrane. Note= A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively. Ref.3

      TISSUE SPECIFICITY: Isoforms 2 and 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level). Ref.3

      DOMAIN:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

      PTM: TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products By similarity.

      Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.9 Ref.10
      Molecular Weight22 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Jurkat cell lysate treated with (+/-) insulin.

      Western Blot Analysis:
      A 1:100~500 dilution of this antibody detected phospho-Bid-S65 on jurkat cell lysate treated with (+/-) insulin.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      AB2994 04053252738913