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ABE2939-25UG Anti-PrimPol

Anti-PrimPol, Cat. No. ABE2939, is a rabbit polyclonal antibody that detects DNA-directed primase/polymerase (PrimPol) and is tested for use in Fluorescence Activated Cell Sorting (FACS), Immunocytochemistry, and Western Blotting.

ABE2939-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE2939-25UG
      DescriptionAnti-PrimPol
      Alternate Names
      • DNA-directed primase/polymerase protein
      • hPrimpol1
      • Coiled-coil domain-containing protein 111
      Background InformationDNA-directed primase/polymerase protein (UniProt: Q96LW4; also known as hPrimpol1, Coiled-coil domain-containing protein 111) is encoded by the PRIMPOL (also known as CCDC111) gene (Gene ID: 201973) in human. PrimPol is an enzyme with both DNA primase and DNA polymerase activities and is involved in DNA damage tolerance. It is detected in nuclei and cytosol with a large fraction localized inside the mitochondria. Upon UV irradiation, it is rapidly redirected to chromatin to reprime DNA synthesis. It associates with nuclear chromatin during the G1 and S phases of unperturbed cell cycles. It contains two RPA1-binding motifs (RBM) (aa 513-527 and 548-556) of which RPA1 is shown to be essential for recruitment to chromatin. It binds to the basic cleft of RPA1. The RPA2-binding motif plays a supporting role in RPA1-binding. PrimPol is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase and is able to bypass certain DNA lesions. It displays high capacity to tolerate DNA damage lesions and is able to synthesize DNA primers downstream of lesions and allows DNA replication to continue. It is also shown to realign primers ahead of 'unreadable lesions' thereby skipping the lesion. In vitro, PrimPol is reported to act as a DNA primase, elongating primers that PrimPol itself synthesizes, or as translesion synthesis DNA polymerase that elongates pre-existing primers across lesions. In murine models PrimPol knockout cells display hypersensitivity to DNA interstrand crosslinks-inducing drugs. Two isoforms of PrimPol have been described that are produced by alternative splicing. (Ref.: Diaz-Talavera, I., et al. (2022). Biomolecules. 12(2); 248; Gonzales-Acosta, D., et al. (2021). EMBO J. 40(14); e106355; Guilliam, TA., et al. (2017). Nat. Commun. 8:15222; Garcia-Gomez, S., et al. (2013). Mol. Cell. 52(4); 541-553; Mouron, S., et al. (2013). Nat. Struct. Mol. Biol. 20(12); 1383-1389).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing Tris-citrate-phosphate buffer (pH 7-8) with 0.09% sodium azide
      Quality LevelMQ200
      Applications
      ApplicationAnti-PrimPol, Cat. No. ABE2939, is a rabbit polyclonal antibody that detects DNA-directed primase/polymerase (PrimPol) and is tested for use in Fluorescence Activated Cell Sorting (FACS), Immunocytochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Fluorescence Activated Cell Sorting (FACS)
      • Immunocytochemistry
      Application NotesTested applications

      Fluorescence Activated Cell Sorting (FACS): A representative lot detected PrimPol in I Fluorescence Activated Cell Sorting applications (Tirman S., et al (2021). Mol Cell. 81(19); 4026-4040).

      Immunocytochemistry Analysis: A representative lot detected PrimPol in Immunocytochemistry application (Tirman S., et al (2021). Mol Cell. 81(19); 4026-4040).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human DNA-directed primase/polymerase (PrimPol).
      EpitopeC-terminal
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects DNA-directed primase/polymerase (PrimPol). It targets an epitope within 14 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRIMPOL
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~65 kDa observed; 64.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U2OS cell lysate.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected PrimPol lysate from U2OS cells, but not in cells with PrimPol knockout.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABE2939-25UG 04065269802310