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MABE342 Anti-Puromycin Antibody, clone 4G11

Anti-Puromycin antibody, clone 4G11 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may also be used with standard immunochemical methods.

Anti-Puromycin Antibody, clone 4G11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins
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MABE342
100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, AWB, FACS, IF, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE342
      DescriptionAnti-Puromycin Antibody, clone 4G11
      Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
      References
      Product Information
      FormatPurified
      Control
      • HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Puromycin antibody, clone 4G11 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may also be used with standard immunochemical methods.
      Key Applications
      • Western Blotting
      • Fluorescence Activated Cell Sorting (FACS)
      • Immunofluorescence
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin. However, a decrease in signal is observed with the addition of Cycloheximide, an inhibitor of protein biosynthesis in eukaryotic organisms.

      Western Blotting Analysis: A representative lot from an independent laboratory detected Puromycin-incorporated neosynthesized proteins in WB (David, A., et al. (2012). J Cell Biol. 197(1):45-57.; Schmidt, E., K., et al. (2009). Nat Methods. 6(4):275-277.).

      Immunofluorescence Analysis: A representative lot from an independent laboratory detected Puromycin-incorporated neosynthesized proteins in IF (David, A., et al. (2012). J Cell Biol. 197(1):45-57.; Schmidt, E., K., et al. (2009). Nat Methods. 6(4):275-277.).

      Fluorescence Activated Cell Sorting Analysis: A representative lot from an independent laboratory detected Puromycin-incorporated neosynthesized proteins in FACS (David, A., et al. (2012). J Cell Biol. 197(1):45-57.; Schmidt, E., K., et al. (2009). Nat Methods. 6(4):275-277.).

      Alexa Fluor® is a registered trademark of Life Technologies®.
      Biological Information
      ImmunogenPuromycin from Streptomyces alboniger
      Clone4G11
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • All
      Species Reactivity NoteDemonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Molecular WeightPuromycin is incorporated in neosynthesized proteins. In the presense of Puromycin only, this antibody detects Puromycin-incorporated neosynthesized proteins at multiple molecular weights. However, a decrease in signal is observed in the co-presense of Cycloheximide, an inhibitor of protein biosynthesis in eukaryotic organisms.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only.

      Western Blotting Analysis: A 1:12,500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HEK293 cell lysates treated with Puromycin only. This antibody also detected small mounts of puromycin in HEK293 cells treated with Puromycin and Cyclohexamide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE342 04053252909221

      Documentation

      Anti-Puromycin Antibody, clone 4G11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Puromycin Antibody, clone 4G11 Certificates of Analysis

      TitleLot Number
      Anti-Puromycin, clone 4G11 3066180
      Anti-Puromycin, clone 4G11 - 2325985 2325985
      Anti-Puromycin, clone 4G11 - 3218872 3218872
      Anti-Puromycin, clone 4G11 - 3289307 3289307
      Anti-Puromycin, clone 4G11 - 3407290 3407290
      Anti-Puromycin, clone 4G11 - 3728337 3728337
      Anti-Puromycin, clone 4G11 - 3826048 3826048
      Anti-Puromycin, clone 4G11 - 3959511 3959511
      Anti-Puromycin, clone 4G11 - 4160990 4160990
      Anti-Puromycin, clone 4G11 - Q2192702 Q2192702
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