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MABC1793-100UG Anti-RNF7 Antibody, clone SAG-10

Anti-RNF7, clone SAG-10, Cat. No. MABC1793, is a mouse monoclonal antibody that detects RNF7 and is tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting.

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MABC1793-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1793-100UG
      DescriptionAnti-RNF7 Antibody, clone SAG-10
      Alternate Names
      • RING-box protein 2
      • Rbx2
      • CKII beta-binding protein 1
      • CKBBP1
      • RING finger protein 7
      • Regulator of cullins 2
      • Sensitive to apoptosis gene protein
      Background InformationRING-box protein 2 (UniProt: Q9UBF6; also known as Rbx2, CKII beta-binding protein 1, CKBBP1, RING finger protein 7, Regulator of cullins 2, Sensitive to apoptosis gene protein) is encoded by the RNF7 (also known as RBX2, ROC2, SAG) gene (Gene ID: 9616) in human. RNF7/RBX2 and RBX1 are members of RING components of Cullin-RING ligases that are required for their enzymatic activity. RBX1 is shown to be constitutively expressed and binds to CUL 1-4; however, RNF7/RBX2 is induced by stress and associates with CUL5 and CUL1. RNF7/RBX2 is expressed in heart, liver, skeletal muscle, and pancreas and is present in cytoplasm and in the nucleus. RNF7/RBX2 is reported to negatively modulate β-TrCP1 levels by shortening its protein half-life by promoting its ubiquitylation via atypical K11-linkage. It can serve as an antioxidant when acting alone or as an E3 ligase when complexed with other components of E3 ubiquitin ligases. It also acts as a survival protein by inhibiting apoptosis induced by a variety of stresses. Overexpression of RNF7/RBX2 is shown to inhibit apoptosis induced by redox, nitric oxide, ischemia, heat shock, and neurotoxins, both in vivo and in vitro. Overexpression of RNF7/RBX2 is observed in multiple human primary tumor tissues and its silencing by siRNA does not affect normal cell growth, but inhibits growth of cancer cells, both in vitro and in vivo by induction of apoptosis. Its silencing is also reported to sensitize radioresistant H1299 and U87 cells to ionizing radiation. (Ref.: Kaung, P., et al. (2016). Sci. Rep. 6; 37441; Jia, L., et al. (2010). Clin Cancer Res; 16(3); 814-824; Tan, M., et al. (2010), Free Rad. Biol. Med. 49(6); 976-983).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-RNF7, clone SAG-10, Cat. No. MABC1793, is a mouse monoclonal antibody that detects RNF7 and is tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesTested Applications

      Western Blotting Analysis: A 1:125 dilution from a representative lot detected RNF7 in H1299 cell lysate.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated RNF7 in Immunoprecipitation application (Kuang, P. et al. (2016). Sci Rep. 6:37441).

      Immunohistochemistry Applications: A representative lot detected RNF7 in Immunohistochemistry applications (Jia, L., et al. (2010). Clin Cancer Res.16(3):814-24).

      Western Blotting Analysis: A representative lot detected RNF7 in Western Blotting applications (Jia, L., et al. (2010). Clin Cancer Res. 6(3):814-24; Tan, M., et al. (2010). Free Radic Biol Med. 49(6):976-83; Kuang, P. et al. (2016). Sci Rep. 6:37441).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 70 amino acids from human Ring finger protein 7 (RNF7).
      EpitopeUnknown
      CloneSAG-10
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone SAG-10 is a mouse monoclonal antibody that specifically detects Ring finger protein 7 (RNF7).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNF7
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~12 kDa observed; 12.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PANC-1 cell lysate.

      Western Blotting Analysis: A 1:125 dilution of this antibody detected RNF7 in PANC-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABC1793-100UG 04065267463711