Millipore Sigma Vibrant Logo

MABS1822-25UG Anti-SHMT Antibody, clone 4D2.1

Anti-SHMT, clone 4D2.1, Cat. No. MABS1822, is a mouse monoclonal antibody that detects mitochondrial Serine hydroxymethyltransferase and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-SHMT Antibody, clone 4D2.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: SHMT2 UniProt Number: P34897
View Products on Sigmaaldrich.com
MABS1822-25UG
25 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1822-25UG
      DescriptionAnti-SHMT Antibody, clone 4D2.1
      Alternate Names
      • Serine hydroxymethyltransferase mitochondrial
      • EC: 2.1.2.1
      • SHMT
      • Glycine hydroxymethyltransferase
      • Serine methylase
      Background InformationSerine hydroxymethyltransferase, mitochondrial (UniProt: P34897; also known as EC: 2.1.2.1, SHMT, Glycine hydroxymethyltransferase, Serine methylase) is encoded by the SHMT2 gene (Gene ID: 6472) in human. SHMT is involved in the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism. Thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA. SHMT catalyzes the reversible conversion of serine and glycine. It uses pyridoxal-5 -phosphate as a cofactor. SHMT is synthesized with a transit peptide (aa 1-29) that is needed for its mitochondrial translocation. In eukaryotes two forms of SHMT have been reported: cytosolic and mitochondrial. In the presence of bound pyridoxal 5'-phosphate it is present in a homotetrameric form and in the absence of pyridoxal 5'-phosphate it exists as a homodimer. Pyridoxal 5'-phosphate binding mediates an important conformation change, which is essential for its tetramerization. Three isoforms of SHMT have been reported that are produced by alternative splicing.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-SHMT, clone 4D2.1, Cat. No. MABS1822, is a mouse monoclonal antibody that detects mitochondrial Serine hydroxymethyltransferase and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution of this antibody from a representative lot detected SHMT in human colon, human liver, and human pancreas tissue sections.

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SHMT in Jurkat cell lysate.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 107 amino acids from the C-terminal half of human mitochondrial Serine hydroxymethyltransferase.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4D2.1 detects human mitochondrial Serine hydroxymethyltransferase. It targets an epitope within 107 amino acids from the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • SHMT2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~56 kDa observed; 55.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SHMT in Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS1822-25UG 04054839543685