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MABS1987 Anti-SREBP-1 Antibody, clone 20B12

Anti-SREBP-1, clone 20B12, Cat. No. MABS1987, a rabbit monoclonal antibody, detects SREBP-1 in human tissues by Western Blotting.

Anti-SREBP-1 Antibody, clone 20B12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Srebf1, Srebp1 UniProt Number: Q9WTN3
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MABS1987
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1987
      DescriptionAnti-SREBP-1 Antibody, clone 20B12
      Alternate Names
      • Sterol regulatory element-binding protein 1
      • SREBP-1
      • Sterol regulatory element-binding transcription factor 1
      Background InformationSterol regulatory element-binding protein 1 (UniProt: Q9WTN3; also known as SREBP-1, Sterol regulatory element-binding transcription factor 1) is encoded by the Srebf1 (also known as Srebp1) gene (Gene ID: 20787) in murine species. SREBPs are a family of transcription factors that regulate lipid homeostasis by controlling the expression of a range of enzymes that are required for endogenous cholesterol, fatty acid, triacylglyceride, and phospholipid synthesis. The three SREBP isoforms known as, SREBP-1a, SREBP-1c, and SREBP-2, have different roles in lipid synthesis. It has been reported that SREBP-1c is mainly involved in fatty acid synthesis and insulin induced glucose metabolism. SREBP-2 is mainly involved in cholesterol synthesis. SREBP-1a isoform is reported to be involved in both of these pathways. Isoform SREBP-1A predominates in spleen, while isoform IC predominates in liver, adrenal gland, brain and adipose tissue and both IA and IC are found in kidney, thymus, testis, muscle, jejunum, and ileum. Expression of SREBP-1C in the liver is reported to be controlled in a circadian manner with a peak at ZT16. SREBPs are synthetized as inactive precursor proteins that are bound to the endoplasmic reticulum membranes and upon activation, the precursor is cleaved off in a two-step process to release the N-terminal active domain in the nucleus. Sterols are shown to inhibit the cleavage of the precursor protein and the mature nuclear form is rapidly catabolized, thereby reducing transcription. SREBP-1 binds to the sterol regulatory element-1 (SRE1 5'-ATCACCCCAC-3'), which flanks the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. Mice overexpressing processed isoform SREBP-1A in adipocytes show enlarged white and brown adipocytes, increased rate of fatty acid synthesis and secretion leading to a fatty liver. Mice lacking isoform SREBP-1C show a lack of up-regulation of several lipogenic enzymes in response to high insulin or LXR activation. (Ref.: Eberle, D et al. (2004) Biochimie 86(11); 839-48).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit monoclonal antibody in cell culture supernatant without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SREBP-1, clone 20B12, Cat. No. MABS1987, a rabbit monoclonal antibody, detects SREBP-1 in human tissues by Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SREBP-1 in 10 µg of HEK293 cell lysates.
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 219 amino acids from the N-terminal region of murine sterol regulatory element-binding protein 1.
      Clone20B12
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityClone 20B12 recognizes SREBP-1 in multiple human tissues.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Srebf1
      • Srebp1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~120 kDa observed; 120.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysates.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SREBP-1 in 10 µg of HepG2 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS1987 04054839193637

      Documentation

      Anti-SREBP-1 Antibody, clone 20B12 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SREBP-1 Antibody, clone 20B12 Certificates of Analysis

      TitleLot Number
      Anti-SREBP-1, clone 20B12 - 3862643 3862643
      Anti-SREBP-1, clone 20B12 - 3995091 3995091
      Anti-SREBP-1, clone 20B12 - 4000692 4000692
      Anti-SREBP-1, clone 20B12 - 4065796 4065796
      Anti-SREBP-1, clone 20B12 - 4134648 4134648
      Anti-SREBP-1, clone 20B12 Monoclonal Antibody 3025851
      Anti-SREBP-1, clone 20B12 Monoclonal Antibody 3106370
      Anti-SREBP-1, clone 20B12 Monoclonal Antibody 2970989
      Anti-SREBP-1, clone 20B12 Monoclonal Antibody Q2834444
      Anti-SREBP-1, clone 20B12 Monoclonal Antibody 3111491