Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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AP1181
Sigma-AldrichAnti-TLR7 Mouse mAb (2C9)
This Anti-TLR7 Mouse mAb (2C9) is validated for use in ELISA, Immunoblotting for the detection of TLR7.
More>>This Anti-TLR7 Mouse mAb (2C9) is validated for use in ELISA, Immunoblotting for the detection of TLR7. Less<<
Anti-TLR7 Mouse mAb (2C9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids of 27-127 human TLR7, expressed as a GST fusion protein
Clone
2C9
Host
Mouse
Isotype
IgG2b
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
19-October-2010 RFH
Synonyms
Anti-Toll-like Receptor 7
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human TLR7 by immunoblotting. Sample: Whole cell lysate from IMR32 cells. Primary antibody: Anti-TLR7 Mouse mAb (2C9) (Cat. No. AP1181) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~120 kDa TLR7 protein.
Background
Stimulation of the NFκB signaling pathway by TLR7 suggests that this protein plays a role in immune response.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 27-127 human TLR7, expressed as a GST fusion protein
Clone
2C9
Isotype
IgG2b
Species
human
Positive control
IMR32 cells
Form
liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
