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MABC1723-100UL Anti-TM4SF1 Antibody, clone 8G4

This mouse monoclonal Anti-TM4SF1, clone 8G4 , Cat. No. MABC1723 is tested for use in Immunocytochemistry, Inhibition, Electron Microscopy, Immunoprecipitation, Western Blotting and Flow Cytometry, for the detection of Transmembrane 4 L6 family member 1.

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MABC1723-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1723-100UL
      DescriptionAnti-TM4SF1 Antibody, clone 8G4
      Alternate Names
      • Transmembrane 4 L6 family member 1
      • Membrane component chromosome 3 surface marker 1
      • Tumor-associated antigen L6
      Background InformationTransmembrane 4 L6 family member 1 (UniProt: P30408; also known as Membrane component chromosome 3 surface marker 1, Tumor-associated antigen L6, TM4SF1) is encoded by the TM4SF1 (also known as M3S1, TAAL6) gene (Gene ID: 4071) in human. TM4SF1 is a multi-pass membrane protein of the L6 tetraspanin family with tetraspanin topology. It has three cytoplasmic domains, four transmembrane domains, and two extracellular domains. It is highly expressed in lung, breast, colon, and ovarian carcinoma. It is highly and selectively expressed on the plasma membranes of tumor cells and on tumor-associated endothelium. It regulates cell motility and proliferation. In PANC-1 and AsPC-1 cells, TM4SF1 is reported to mediate the expression and activities of matrix metallopeptidase 2 (MMP2) and matrix metallopeptidase 9 (MMP9), which are considered as the major regulators of extracellular matrix degradation to increase the cells migration and invasion. Clone 8G4 is reported to be highly effective in reducing blood vessel formation in Matrigel plugs and is shown to destroy PC3 cells and vascular components incorporated into the Matrigel mixture. (Ref.: Yang, J., et al. (2017). Sci. Rep. 7; Article 45895; Sciuto, TE., et al. (2015). Biochem. Biophys. Res. Commun. 465(3): 338 343; Lin, C., et al. (2014). Angiogenesis. 17(4); 897-907).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-TM4SF1, clone 8G4 , Cat. No. MABC1723 is tested for use in Immunocytochemistry, Inhibition, Electron Microscopy, Immunoprecipitation, Western Blotting and Flow Cytometry, for the detection of Transmembrane 4 L6 family member 1.
      Key Applications
      • Immunocytochemistry
      • Inhibition
      • Electron Microscopy
      • Immunoprecipitation
      • Western Blotting
      • Flow Cytometry
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected TM4SF1 in Flow Cytometry applications (Lin, C.I., et. al. (2014). Angiogenesis. 17(4):897-907).

      Western Blotting Analysis: A representative lot detected TM4SF1 in Western Blotting applications (Sciuto, T.E., et. al. (2015). Biochem Biophy Res Commun. 465(3):338-443).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated TM4SF1 in Immunoprecipitation applications (Sciuto, T.E., et. al. (2015). Biochem Biophy Res Commun. 465(3):338-443).

      Immunocytochemistry Analysis: A representative lot detected TM4SF1 in Immunocytochemistry applications (Lin, C.I., et. al. (2014). Angiogenesis. 17(4):897-907).

      Electron Microscopy: A representative lot detected TM4SF1 in Electron Microscopy applications (Sciuto, T.E., et. al. (2015). Biochem Biophy Res Commun. 465(3):338-443).

      Inhibition Analysis: A representative lot blocked the formation new blood vessels in Matrigel plugs. (Lin, C.I., et. al. (2014). Angiogenesis. 17(4):897-907).


      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHuman umbilical vein endothelial cells (HUVEC) overexpressing human TM4SF1.
      EpitopeExtracellular domain
      Clone8G4
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 8G4 is a mouse monoclonal antibody that detects human Transmembrane 4 L6 family member 1 (TM4SF1). It targets an epitope within the extracellular loop 2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TM4SF1
      • M3S1
      • TAAL6
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight21.63 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in human umbilical vein endothelial cells (HUVEC).

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected TM4SF1 in human umbilical vein endothelial cells (HUVEC).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABC1723-100UL 04065266298345