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ABN1721-25UG Anti-TMEM100

Anti-TMEM100, Cat. No. ABN1721, is a highly specific rabbit polyclonal antibody that targets Transmembrane protein 100 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Research Category: Neuroscience Gene Symbol: Tmem100 UniProt Number: Q9CQG9
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ABN1721-25UG
25 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIF, IH(P), IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1721-25UG
      DescriptionAnti-TMEM100
      Alternate Names
      • Transmembrane protein 100
      Background InformationTransmembrane protein 100 (UniProt: Q9CQG9; also known as TMEM100) is encoded by the Tmem100 gene (Gene ID: 67888) in murine species. TMEM100 is a multi-pass membrane protein that is shown to be involved in renal development, vasculogenesis and lung cancer invasiveness. It plays a role during embryonic arterial endothelium differentiation and vascular morphogenesis through the ACVRL1 receptor-dependent signaling pathway upon stimulation by bone morphogenetic proteins, such as GDF2/BMP9 and BMP10. TMEM100 is also involved in the regulation of nociception by forming a complex with TRPA1 and TRPV1 and act as a modulator of their interaction in dorsal root ganglia (DRG) neurons. It is reported to weaken TRPA1 and TRPV1 interaction, thereby releasing the inhibition of TRPA1 by TRPV1 and increasing the single-channel open probability of the TRPA1-TRPV1 complex. TMEM100-deficient mice show a reduction in inflammatory mechanical hyperalgesia and TRPA1- but not TRPV1-mediated pain. A mutation involving aa 117-119 KRR to QQQ is shown to abolish TMEM100 interaction with TRPA1, but increases interaction with TRPV1. Mutations is Tmem100 gene can lead to embryonic lethality due to impaired differentiation of arterial endothelium and defects of vascular morphogenesis. (Ref.: Weng, H-J., et al. (2015). Neuron 85(4), 833-846).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-TMEM100, Cat. No. ABN1721, is a highly specific rabbit polyclonal antibody that targets Transmembrane protein 100 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected TMEM100 in mouse colon, rat peripheral nerve, and human cerebral cortex tissues.
      Immunofluorescence Analysis: A representative lot detected TMEM100 in DRGs (Weng, H.J., et. al. (2015). Neuron. 85(4):833-46).
      Western Blotting Analysis: A representative lot detected TMEM100 in DRG from cervical to lumbar levels (Weng, H.J., et. al. (2015). Neuron. 85(4):833-46).
      Immunoprecipitation Analysis:  representative lot detected TMEM100 in whole-cell DRG or CHO cells lysates (Weng, H.J., et. al. (2015). Neuron. 85(4):833-46).
      Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected TMEM100 in Dorsal root ganglion (DRG) of WT & TMEM100 CKO mice  (Courtesy of Drs. Hao-Jui Weng and Xinzhong Dong at Johns Hopkins University).
      Immunofluorescence Analysis: A 1:200 dilution from a representative lot detected TMEM100 in Dorsal root ganglion (DRG) sections from Avil-Cre;Tmem100+/+ (Wild Type mice) and Avil-Cre;Tmem100fl/fl (Tmem100 CKO) mice   (Courtesy of Drs. Hao-Jui Weng and Xinzhong Dong at Johns Hopkins University).
       
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminus region of murine Transmembrane protein 100 (TMEM100).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects transmembrane protein 100 (TMEM100) in murine cells. It targets an epitope with the 19 amino acids from the N-terminal end.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • Tmem100
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~17 kDa observed; 14.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse lung tissue lysate.
      Western Blotting Analysis: 1 µg/mL of this antibody detected TMEM100 in 10 µg of mouse lung tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABN1721-25UG 04054839490064

      Documentation