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AB9913 Anti-TNRC6B Antibody

Anti-TNRC6B Antibody is a Rabbit Polyclonal Antibody for detection of TNRC6B also known as Trinucleotide repeat-containing gene 6B protein & has been validated in WB, ICC & IP.

Anti-TNRC6B Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: TNRC6B, KIAA1093 UniProt Number: Q9UPQ9
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AB9913
100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, OxWB, ICC, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9913
      DescriptionAnti-TNRC6B Antibody
      Alternate Names
      • Trinucleotide repeat-containing gene 6B protein
      Background InformationTNRC6B (trinucleotide repeat containing 6B) is a argonaute-associated protein that localizes to cytoplasmic P bodies and is essential for control of miRNA-guided mRNA cleavage. TNRC6B is one of the mammalian homologs of the metazoan GW182 and is required for well-organized miRNA repression. Studies suggest that TNRC6B-mediated miRNA silencing is promoted by the C-terminal silencing domain.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-TNRC6B Antibody is a Rabbit Polyclonal Antibody for detection of TNRC6B also known as Trinucleotide repeat-containing gene 6B protein & has been validated in WB, ICC & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected TNRC6B in HeLa cells. (Zipprich, J., et al. (2009). RNA. 15:781–793.)
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human TNRC6B.
      EpitopeN-terminan domain of TNRC6B
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAntibody recognizes TNRC6B
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Ox
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TNRC6B
      • KIAA1093
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent translational repression and siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. Ref.7 Ref.11 Ref.12

      SUBUNIT STRUCTURE: Interacts with EIF2C1/AGO1, EIF2C2/AGO2, EIF2C3/AGO3 and EIF2C4/AGO4. Ref.7 Ref.11 Ref.12 Ref.9

      SUBCELLULAR LOCATION: Cytoplasm › P-body. Note: Mammalian P-bodies are also known as GW bodies (GWBs). Ref.7 Ref.11

      SEQUENCE SIMILARITIES: Belongs to the GW182 family.

      Contains 1 RRM (RNA recognition motif) domain.

      SEQUENCE CAUTION: The sequence BAA83045.2 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~150/190 kDa observed. (Meister, G., et al. (2005). Current Biology. 15:2149–2155). An uncharacterized band at ~39 kDa may be observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected TNRC6B on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      AB9913 04053252685071

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies