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MABE1135-100UG Anti-TRF2 Antibody, clone T2JKM3

Anti-TRF2, clone T2JKM3, Cat. No. MABE1135, is a highly specific mouse monoclonal antibody that targets Telomeric repeat-binding factor 2 (TRF2) and has been tested for use in Immunocytochemistry and Western Blotting.

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MABE1135-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1135-100UG
      DescriptionAnti-TRF2 Antibody, clone T2JKM3
      Alternate Names
      • Telomeric repeat-binding factor 2
      • TTAGGG repeat-binding factor 2
      • Telomeric DNA-binding protein
      • TERF2
      Background InformationTelomeric repeat-binding factor 2 (UniProt: Q15554; also known as TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein, TERF2) is encoded by the TERF2 (also known as TRBF2, TRF2) gene (Gene ID: 7014) in human. TERF2 is homodimeric protein that is ubiquitous in its expression. Higher expression levels are reported in spleen, thymus, prostate, uterus, testis, peripheral blood leukocytes, colon, and small intestine. Two soforms of TERF2 are reported that are produced by alternative splicing.TERF2 binds to telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. It is also required to recruit a number of factors and enzymes required for telomere protection, including the sheltering complex, TERF2IP/RAP1 and DCLRE1B/Apollo. TEFR2 colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase. It is phosphorylated upon DNA damage and the phosphorylated TERF2 is not bound to telomeric DNA, and rapidly localizes to damage sites. Inhibition of TRF2 causes apoptosis in several mammalian cell types and its over-expression is observed in a variety of human cancers.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-TRF2, clone T2JKM3, Cat. No. MABE1135, is a highly specific mouse monoclonal antibody that targets Telomeric repeat-binding factor 2 (TRF2) and has been tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot detected TRF2 in IMR90 primary human fibroblasts.

      Western Blotting Analysis: A 1:200 dilution from a representative lot detected TRF2 in HT1080 cell lysate.
      Biological Information
      ImmunogenTagged full length recombinant human Telomeric repeat-binding factor 2.
      CloneT2JKM3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone T2JKM3 detects Telomeric repeat-binding factor 2 in human cells.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TERF2
      • TRF2
      • TRBF2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~60 kDa observed; 59.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HT1080 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected TRF2 in HT1080 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE1135-100UG 04054839468957

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