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MABT1335-25UG Anti-Tropomyosin 3 Antibody, clone 2G10.2

Anti-Tropomyosin 3, clone 2G10.2, Cat. No. MABT1335, is a mouse monoclonal antibody that detects Tropomyosin Tpm3.1 and Tpm3.2 and has been tested for use in Immunofluorescence and Western Blotting.

MABT1335-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1335-25UG
      DescriptionAnti-Tropomyosin 3 Antibody, clone 2G10.2
      Alternate Names
      • Gamma-tropomyosin
      • Tropomyosin-3
      • Tropomyosin-5
      • TPM3
      • Tm3
      Background InformationTropomyosin alpha-3 chain (UniProt: Q63610; also known as Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5) is encoded by the Tpm3 (also known as Tpm-5, Tpm5) gene (Gene ID: 117557) in rat. The TPM3 gene codes for the slow-twitch skeletal muscle isoform ( s Tm) and at least 9 LMW cytoskeletal isoforms referred to as Tm5NM1 to Tm5NM11. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. In non-muscle cells tropomyosins are implicated in stabilizing cytoskeleton actin filaments. Tropomyosins have been implicated in the pathogenesis of cancer where high molecular weight isoforms are consistently down-regulated in transformed cells, while malignant cells display an increased reliance on low molecular weight isoforms. Tropomyosin 3 is a homodimeric protein that can form heterodimers with a beta (TPM2) chain. Its coiled coil structure is formed by 2 polypeptide chains. (Ref.: Glass, JJ et al. (2015). BMC Cancer. 15; 712).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Tropomyosin 3, clone 2G10.2, Cat. No. MABT1335, is a mouse monoclonal antibody that detects Tropomyosin Tpm3.1 and Tpm3.2 and has been tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected Tropomyosin 3 in human lung tissue lysate.

      Immunofluorescence Analysis: A representative lot detected Tropomyosin 3 in Immunofluorescence applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).

      Western Blotting Analysis: A representative lot detected Tropomyosin 3 in Western Blotting applications (Schevzov, G., et. al. (2011). Bioarchitecture. 1(4):135-164).
      Biological Information
      ImmunogenDiphtheria Toxoid Carrier Protein-conjugated linear peptide corresponding to 27 amino acids from the C-terminal region of rat Tpm3.1 and Tpm3.2.
      EpitopeC-Terminus of Tpm 3.1 and Tpm 3.2
      Clone2G10.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2G10.2 is a mouse monoclonal antibody that specifically detects Tpm3.1 and Tpm3.2. It targets an epitope within 27 amino acids from the C-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TPM3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~30 kDa observed; 29.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse liver tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Tropomyosin 3 in mouse liver tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABT1335-25UG 04054839029103

      Documentation

      Anti-Tropomyosin 3 Antibody, clone 2G10.2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tropomyosin 3 Antibody, clone 2G10.2 Certificates of Analysis

      TitleLot Number
      Anti-Tropomyosin 3, clone 2G10.2 - 3277770 3277770

      References

      Reference overviewPub Med ID
      Tropomyosin isoforms and reagents
      Galina Schevzov 1 , Shane P Whittaker, Thomas Fath, Jim Jc Lin, Peter W Gunning
      Bioarchitecture  1(4)  135-164  2011

      Show Abstract       		22069507 22069507