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CBL1300 Anti-VCAM-1 Antibody, clone M/K

Anti-VCAM-1 Antibody, clone M/K is an antibody against VCAM-1 for use in FC, IH & IP.

Anti-VCAM-1 Antibody, clone M/K MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: VCAM1, INCAM-100, MGC99561, CD106, DKFZp779G2333, L1CAM UniProt Number: P19320
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CBL1300
500 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, IHC, IP, FUNCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL1300
      ReplacesMAB2242
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-VCAM-1 Antibody, clone M/K
      Alternate Names
      • CD106
      References
      Product Information
      FormatPurified
      Control
      • EOMA and BMS2 endothelial cell lysates
      PresentationThe monoclonal is presented in phosphate buffered saline containing 10mM sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-VCAM-1 Antibody, clone M/K is an antibody against VCAM-1 for use in FC, IH & IP.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      • Affects Function
      Application NotesStudies of cell-cell interaction

      Cell adhesion assays

      Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures

      Immunoprecipitation of the VCAM-1 integrin complex

      Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      CloneM/K - 2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificityRecognizes Murine vascular adhesion molecule (VCAM-1), single chain glycoprotein of Mr 100- kDa (under reducing conditions). VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. The antibody reacts with all known isoforms of murine VCAM-1.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
      Gene Symbol
      • VCAM1
      • INCAM-100
      • MGC99561
      • CD106
      • DKFZp779G2333
      • L1CAM
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19320 # Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
      SIZE: 739 amino acids; 81276 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
      DOMAIN: SwissProt: P19320 Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
      PTM: Sialoglycoprotein.
      DISEASE:SwissProt: P19320 # May play an important role in the genesis of atherosclerosis and rheumatoid arthritis.
      SIMILARITY: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      CBL1300 04053252362606

      Documentation

      Anti-VCAM-1 Antibody, clone M/K SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-VCAM-1 Antibody, clone M/K Certificates of Analysis

      TitleLot Number
      RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) - 3538957 3538957
      RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY 3128869
      RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY 2931117
      RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 2179338 2179338
      RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 2318097 2318097
      RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 2697525 2697525
      RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 3046723 3046723
      RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 3890519 3890519
      RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY - 3930367 3930367
      RAT ANTI-MOUSE CD 106 (VCAM-1) (UNLABELLED, PURIFIED) MONOCLONAL ANTIBODY -2554907 2554907
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies