Millipore Sigma Vibrant Logo

ABS1656 Anti-VPS4B (UT292)

Detect Vacuolar protein sorting-associated protein 4B using this rabbit polyclonal Anti-VPS4B (UT292), Cat. No. ABS1656, validated for use in Immunocytochemistry and Western Blotting.

Anti-VPS4B (UT292) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: VPS4B, SKD1, SKD1B, VPS4-2, VPS42, MIG1 UniProt Number: O75351
View Products on Sigmaaldrich.com
ABS1656
50 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1656
      DescriptionAnti-VPS4B (UT292)
      Alternate Names
      • Vacuolar protein sorting-associated protein 4B
      • Cell migration-inducing gene 1 protein
      • Protein SKD1
      • Suppressor of K(+) transport growth defect 1
      Background InformationVacuolar protein sorting-associated protein 4B (EC 3.6.4.6; UniProt O75351; also known as Cell migration-inducing gene 1 protein, Protein SKD1, Suppressor of K(+) transport growth defect 1) is encoded by the VPS4B (also known as SKD1, SKD1B, VPS4-2, VPS42, MIG1) gene (Gene ID 9525) in human. SKD1 is a ubiquitously expressed protein that is involved in intracellular protein trafficking at the late endosomal protein transport step. Displays about 80% sequence homology with VPS4A. It recognizes membrane-associated ESCRT-III (endosomal sorting complex required for transport III) assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Also, redistributes the ESCRT-III components to the cytoplasm for further rounds of multi-vesicular body (MVB) sorting. In conjunction with the ESCRT machinery, it also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan. SKD1 is proposed to be monomeric or homodimeric in nucleotide-free form and to oligomerize upon binding to ATP to form two stacked hexameric or heptameric rings with a central pore through which ESCRT-III substrates are translocated in an ATP-dependent manner.

      Ref.: Scheuring, S et al. (2001). J. Mol. Biol. 312, 469-480.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Vacuolar protein sorting-associated protein 4B using this rabbit polyclonal Anti-VPS4B (UT292), Cat. No. ABS1656, validated for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected VPS4B immunoreactivity concentrated at the centrosome during mitosis and at the midbody during cytokinesis in 3% paraformaldehyde-fixed HeLa cells. No staining was seen in VPS4B shRNA-transfected HeLa cells (Morita, E., et al. (2010). Proc. Natl. Acad. Sci. U.S.A. 107(29):12889-12894).

      Western Blotting Analysis: A representative lot detected the VPS4B target band in lysate from untransfected, but not VPS4B shRNA-transfected HeLa cells (Morita, E., et al. (2010). Proc. Natl. Acad. Sci. U.S.A. 107(29):12889-12894).
      Biological Information
      ImmunogenHis-tagged recombinant full-length human VPS4B.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityVPS4B immunostaining and target band detection by UT292 was observed only in untransfected, but not VPS4B shRNA-transfected HeLa cells (Morita, E., et al. (2010). Proc. Natl. Acad. Sci. U.S.A. 107(29):12889-12894).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VPS4B
      • SKD1
      • SKD1B
      • VPS4-2
      • VPS42
      • MIG1
      Purification MethodUnpurified.
      UniProt Number
      Molecular Weight~52 kDa observed. 49.30 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected VPS4B in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS1656 04054839135293